苦皮藤素V在粘虫和小地老虎幼虫体内的穿透与代谢

采用高效液相色谱技术研究了苦皮藤素Ⅴ在粘虫Mythimna separata和小地老虎Agrotis ypsilon幼虫中的穿透及代谢。结果表明:苦皮藤素V均不能从粘虫和小地老虎幼虫的体壁穿透到血腔或从血腔穿透到中肠,但很容易从中肠穿透到血腔,且穿透速率无差异;苦皮藤素V在小地老虎幼虫体内的代谢解毒速率远大于其在粘虫幼虫体内的代谢速率,其半衰期分别为5.5和13.1 h。本研究结果表明,苦皮藤素V对粘虫和小地老虎幼虫的选择毒杀作用与药物的穿透能力无关,其在试虫体内的解毒代谢差异才是其对昆虫具有选择作用的机理之一。

Investigation on the penetration and metabolism of celangulin V in the larvae of Mythimna separata and Agrotis ypsilon

Penetration and metabolism of celangulin V in the larvae of Mythimna separata and Agrotis ypsilon were studied using high performance liquid chromatography. The results showed that celangulin V can not penetrate into the haemolymph from the body wall or penetrate into the midgut from the haemolymph in both of larvae. Whereas, celangulin V with the same rate of penetration is easily penetrate into the haemolymph from the midgut in the case of both larvae. The metabolic rate of celangulin V in the larvae of A.ypsilon is greater than that in the larvae of M. separata and the half-life of celangulin V was 5.5 h and 13.1 h in the larvae of A.ypsilon and M. separata, respectively. The results indicate that the penetration was not involved in the selective toxicity of celangulin V, whereas, detoxification metabolism in the larvae of A.ypsilon and M. separata was one of the mechanisms of this selective toxicity.