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锡兰肉桂丙酮提取物对棉铃虫的毒力及其代谢酶活性的影响
引用本文:陈青,覃丽金,杨卫帆,吴忠琴.锡兰肉桂丙酮提取物对棉铃虫的毒力及其代谢酶活性的影响[J].农药学学报,2004,6(1):41-45.
作者姓名:陈青  覃丽金  杨卫帆  吴忠琴
作者单位:1.中国热带农业科学院 环境与植物保护研究所,海南 儋州 571737
基金项目:中国热带农业科学院科技基金(Ryky0151)资助.
摘    要:采用叶片浸渍法和紫外分光光度法研究了锡兰肉桂Cinnamomum zexlanicum Nees丙酮提取物对棉铃虫Heliothis armigera (Hübner)的毒力及其代谢酶活性的影响,旨在为进一步深入了解锡兰肉桂的杀虫活性 及其在有害生物防治中的开发应用提供理论依据。试验结果表明,锡兰肉桂丙酮提取物对棉铃虫具有较强的毒杀作用,处理后72 h的LC50和LC95值分别为5.16 mg/mL和27.26 mg/mL,其13.33 mg/mL对棉铃虫的48 h和72 h校正死亡率分别为69.8%和80.2%,其6.67 mg/mL对棉铃虫的72 h校正死亡率为64.0%。锡兰肉桂丙酮提取物对棉铃虫酯酶、羧酸酯酶、酸性磷酸酯酶和碱性磷酸酯酶均具有明显的抑制作用,作用大小随处理浓度增加而增强。

关 键 词:锡兰肉桂Cinnamomum  zexlanicum  Nees    丙酮提取物    棉铃虫    Heliothis  armigera(Hübner)    毒力    代谢酶
文章编号:1008-7303(2004)01-0041-05
收稿时间:2003/10/27 0:00:00
修稿时间:2003年10月27

The Toxicity of Cinnamomum zexlanicum Nees Acetone Extract and its Effects on Several Esterases in Heliothis armigera (Hübner)
CHEN Qing,QIN Li-jin,YANG Wei-fan and WU Zhong-qin.The Toxicity of Cinnamomum zexlanicum Nees Acetone Extract and its Effects on Several Esterases in Heliothis armigera (Hübner)[J].Chinese Journal of Pesticide Science,2004,6(1):41-45.
Authors:CHEN Qing  QIN Li-jin  YANG Wei-fan and WU Zhong-qin
Institution:1.Environment and Plant Protection Research Institute, CATAS, Danzhou 571737,China
Abstract:The toxicity of acetone extract of Cinnamomum zexlanicum Nees and its effects on several esterases in Heliothis armigera (Hübner) were studied by leaf immersion method and ultraviolet spectrophotometry. The results showed that the acetone extract of C.zexlanicum had a good activity to H. armigera. The LC50 values and the LC95 values were 5.16 mg/mL and 27.26 mg/mL after 72 h of treatment; the corrected mortality were 69.8% and 80.2% after 48 h and 72 h of treatment at 13.33 mg/mL concentration and the corrected mortality was 64.0% after 72 h of treatment at 6.67 mg/mL concentration. The results also indicated that acetone extract of C. zexlanicum significantly inhibited esterase, carboxylesterase, alkaline phosphate esterase and acid phosphate esterase activities of H. armigera under various acetone extract concentrations of 5.0 ,4.0 ,3.0 ,2.0 and 1.0 μg/mL;the higher the treatment concentration, the heavier the inhibition.
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