超高效液相色谱-串联质谱法测定莲雾中多菌灵、噻菌灵和甲霜灵残留

建立了莲雾中多菌灵、噻菌灵和甲霜灵残留的超高效液相色谱-串联质谱(UPLC-MS/MS)的快速检测方法。样品直接用乙腈匀浆提取,过0.22μm滤膜后,在电喷雾正离子模式下以多反应监测(MRM)模式测定,基质匹配标准溶液外标法定量。结果表明:方法的线性范围为0.01~0.5μg/m L,决定系数(R2)0.99;在0.04、0.4和2 mg/kg 3个添加水平下,方法的平均回收率为75%~107%,相对标准偏差为2.0%~8.9%(n=5),定量限均为0.04 mg/kg。该方法样品前处理简单、快速,分析时间短,灵敏度、准确度和精密度均符合农药残留检测要求。

Determination of carbendazim,thiabendazole and metalaxyl residues in wax-apple by ultra performance liquid chromatography-tandem mass spectrometry

A rapid method for determining carbendazim, thiabendazole and metalaxyl residue in wax-apples was developed with ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) detection. The samples were extracted with acetonitrile. The samples were detected by UPLC-MS/MS under positive electrospray ionization and multiple reaction monitoring mode. The matrix-matched external standard calibration curves were used for the quantitative analysis. It was shown that the calibration curves were linear in the range of 0.01-0.5 μg/mL and coefficient of determination R2>0.99.The limits of quantitation were 0.04 mg/kg. The average recoveries of the three pesticides were 75%-107% at spiked levels of 0.04, 0.4 and 2 mg/kg, with relative standard deviations in the range of 2.0%-8.9% (n=5). The sample pretreatment of the method is simple and time-saving. The method is applied for detection of carbendazim, thiabendazole and metalaxyl residues in market wax-apples.