用蛋白核小球藻(Chlorella pyrenoidosa)评价除草剂活性的微型筛选方法研究

首次在国内以蛋白核小球藻(Chlorella pyrenoidosa) 为模式生物对24 种除草剂进行了生物筛选试验, 结果表明, 用蛋白核小球藻筛选除草剂活性的方法快速、敏感, 恰好弥补了培养皿法的不足之处, 可以构成除草剂活性筛选的第二套筛子。用这种方法对化合物进行常规筛选, 将减少具有潜在活性化合物的漏筛, 也可作为初筛以适应微型高通量筛选的要求。

Study on Microscreening Method to Evaluate Herbicidal Activity Using Chlorella pyrenoidosa

The biological screening for 24 herbicides in 15 different chemical classes with 9 different functions using Chlorella pyrenoidosa as a model biology, shows that this method for herbicide screening is very quick, simple,sensitive and more perfect than the Petri dishes method.Thus, the in vitro system can offer a second screening sieve to ensure that new potential lead structures will not be lost. Moreover, a set of biotests using Chlorella pyrenoidosa cell suspensions is suggested to form a herbicide prescreen in order to eliminate inactive compounds before the more expensive greenhouse screen on higher plants. The method has been shown to be suitable for the requirements for a microscreen and high throughput screening in vitro (HTS in vitro )