利用HPLC检测禾谷丝核菌菌丝对戊唑醇的吸收

建立了禾谷丝核菌Rhizoctonia cerealis菌丝吸收戊唑醇的反相高效液相色谱(HPLC)检测方法,样品经萃取净化后过HPLC检测,外标法定量。方法的最小检出量和最低检出浓度分别为0.2 ng和0.01 mg/kg,回收率及精密度高,能满足真菌抗药性机制研究中定量分析的要求。与传统的液体闪烁计数法相比,该方法成本低、简便、准确。同时,比较了用戊唑醇处理后,敏感菌株和不同抗性水平菌株对戊唑醇的吸收及药剂在菌株体内的积累情况。结果表明,在0.5 mg/L剂量下,戊唑醇在抗性菌株体内的积累浓度要小于在敏感菌株体内,处理后24 h,敏感菌株体内戊唑醇的浓度要比高抗菌株高127.74%,比两个中抗菌株也分别高47.72%和7.83%。

Determination of the Absorption of Tebuconazole by Mycelium of Rhizoctonia cerealis with High Performance Liquid Chromatography

The quantitative analysis method of high performance liquid chromatography(HPLC) was established to determine the absorption of tebuconazole by mycelium of Rhizoctonia cerealis.After the sample was extracted and purified,it was determined by the HPLC and the external standard method was used.The minimum detectable amount and concentration were 0.2 ng and 0.01 mg/kg,(respectively).The recovery and(precision) were high.It could completely satisfy with the demands of quantitative analysis on the study of drugfast mechanism of fungi.The method has some merits(including) low cost,simple and accurate,compared with traditional liquid scintillation method.The comparison of the absorption of tebuconazole was made between resistant-strains and sensitive-strain.The(results) showed that after treated with tebuconazole (0.5 mg/L),the amount of tebuconazole(accumulated) by resistant-strains was lower than that by sensitive-strain.After 24 hours,the amount of tebuconazole accumulated by sensitive-strain was 127.74% higher than that of high resistant-strain,47.72% and 7.83% higher than that of middle resistant-strains,respectively.