| 苍白杆菌B2对甲基对硫磷降解途径研究 |
| |
| 引用本文： | 柏文琴,李梅,邱星辉,何凤琴,冷欣夫.苍白杆菌B2对甲基对硫磷降解途径研究[J].农药学学报,2004,6(4):48-54. |
| |
| 作者姓名： | 柏文琴 李梅 邱星辉 何凤琴 冷欣夫 |
| |
| 作者单位： | 1.中国科学院 动物研究所, 农业虫害鼠害综合治理研究国家重点实验室 北京 100080 |
| |
| 基金项目： | 中国科学院院长基金特别支持项目(残留农药的酶降解技术研究)资助. |
| |
| 摘 要： | 采用薄层层析、紫外吸收光谱以及气谱-质谱联用的方法分析了苍白杆菌Ochrobacterum sp. B2降解甲基对硫磷的中间产物。结果表明,B2水解甲基对硫磷产生对硝基苯酚(PNP),PNP通过产生4-硝基邻苯二酚(4-nitrocatechol,4-NC)和1,2,4-苯三酚(1,2,4-benzenetriol) 的途径进一步代谢。苍白杆菌B2可以以PNP和4-NC为碳源生长,在菌的初始OD600值为0.02时,B2可在48 h内将50 mg/L的PNP完全降解, 在60 h内,将30 mg/L的4-NC完全降解。
|
| 关 键 词： | 苍白杆菌 甲基对硫磷 对硝基苯酚 4-硝基邻苯二酚 1 2 4-苯三酚 降解 |
| 文章编号： | 1008-7303(2004)04-0048-07 |
| 收稿时间： | 7/9/2004 12:00:00 AM |
| 修稿时间： | 2004年7月9日 |
Degradative Pathway of Parathion-Methyl by Ochrobacterum sp. B2 |
| |
| BAI Wen-qin,LI Mei,QIU Xing-hui,HE Feng-qin and LENG Xin-fu.Degradative Pathway of Parathion-Methyl by Ochrobacterum sp. B2[J].Chinese Journal of Pesticide Science,2004,6(4):48-54. |
| |
| Authors: | BAI Wen-qin LI Mei QIU Xing-hui HE Feng-qin and LENG Xin-fu |
| |
| Institution: | 1.State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, the Chinese Academy of Sciences, Beijing 100080, China2.Graduate School of the Chinese Academy Sciences, Beijing 100039, China |
| |
| Abstract: | The parathion-methyl degradation by Ochrobacterum sp. B2 was analyzed by thin layer (chromatography,) UV spectroscopy and gas chromatography-mass spectrometry analysis. The (results) indicated that parathion-methyl was hydrolyzed to p-nitrophenol, and this metabolite was further (degraded) to 4-nitrocatechol and 1,2,4-benzenetriol. B2 could grow on a medium with p-(nitrophenol) or 4-nitrocatechol as a sole carbon source. 50 mg/L p-(nitrophenol) was degraded (completely) in 48 h and 30 mg/L 4-nitrocatechol disappeared almost in 60 h when the (initial) OD_(600) of B2 cells was 0.02. |
| |
| Keywords: | Ochrobacterum sp parathion-methyl p-nitrophenol 4-nitrocatechol 1 2 4-(benzenetriol ) degradation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《农药学学报》浏览原始摘要信息 |
| 点击此处可从《农药学学报》下载免费的PDF全文 |
|
