工业废水对黄瓜幼苗生长及叶片抗氧化系统的影响

采用以甘肃省境内某地区化肥厂、造纸厂的工业废水以及此两厂的混合废水作溶剂的培养液(分别记作废液1、废液2和废液3)培养黄瓜幼苗,研究了其对黄瓜幼苗的生长及其叶组织中活性氧清除系统的影响。结果发现,(1)培养5 d后,生长在废液1中的幼苗,叶片组织中的几种抗氧化酶除APX的活性显著增加外(P<0.01),CAT、SOD和GR活性均无明显变化(P>0.05)。生长在废液2中的CAT和GR的活性基本未变,APX和SOD的活性分别在P<0.01和P<0.05的水平上增加。在废液3中,CAT和SOD及APX的活性分别在P<0.05和P<0.01的水平上增加,而GR活性降低(P<0.05)。(2)培养13 d后,无论在哪种废水中,黄瓜幼苗的伸长生长和干物质积累及叶片组织中APX、SOD、CAT和GR活性、GSH和ASA含量均明显降低(P<0.05或P<0.01),H2O2、O2、MDA含量和电解质泄漏率明显增加(P<0.05或P<0.01)。结果表明,化肥厂、造纸厂的工业废水以及此两厂的混合废水对黄瓜幼苗叶组织中的抗氧化系统有明显的破环作用,最终影响幼苗的生长。

Effects of nutriment solution with industrial wastewater on antioxidant system in leaf tissues of cucumber seedlings

The effects of wastewater on the antioxidant system in leaf tissues of cucumber seedlings were studied by culturing with different solutions named waste solution 1(S1),waste solution 2(S2) and waste solution 3(S3),in which the solutes were the necessary nutriments of plants and the solvents were the wastewater from paper mill,the wastewater from chemical fertilizer factory and the mixture of both origins.It was found that after being cultured for 5 days,in the leaf tissues,the activities of APX in S1,APX and SOD in S2,CAT,SOD and APX in S3 were increased (P<0.01 or P<0.05),and the activity of GR in S3 was decreased (P<0.05). After being cultured for 13 days,in the leaf tissues,the activities of APX,SOD,CAT and GR and the content of GSH and ASA were decreased (P<0.01 or P<0.05) in these three kinds of waste solution.At the same time,the contents of H₂O₂,O^·₂ and MDA and electrolyte leakage rate in the leaf tissues were increased.The results showed that the three kinds of waste solution were all harmful to the antioxidant system in the leaf tissue of cucumber seedlings.In despite of being given all necessary nutriments,the growth of cucumber seedlings was remarkably inhibited by the waste solutions.