| 北方旱寒区冬油菜抗寒性相关指标的QTL定位 |
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| 引用本文： | 武军艳,孔德晶,方彦,孙万仓,刘海卿,刘自刚,李学才.北方旱寒区冬油菜抗寒性相关指标的QTL定位[J].干旱地区农业研究,2017,35(1):205-210. |
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| 作者姓名： | 武军艳 孔德晶 方彦 孙万仓 刘海卿 刘自刚 李学才 |
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| 作者单位： | 甘肃农业大学农学院，甘肃省作物遗传改良与种质创新重点实验室，甘肃省干旱生境作物学重点实验室，甘肃 兰州730070 |
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| 基金项目： | 甘肃省青年科技基金计划(145RJYA253)；农业部产业技术体系建设资金项目(CARS-13)；甘肃省自然科学基金项目 |
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| 摘 要： | 以抗寒性不同的白菜型冬油菜品种陇油7号与陇油9号为亲本构建的F2群体为材料,对白菜型冬油菜抗寒性相关的生理指标进行了QTL定位分析。在白菜型油菜的10条染色体上定位了SOD活性、POD活性、CAT活性、MDA含量、游离脯氨酸含量和可溶性蛋白含量共6个性状的24个QTL位点,可解释表型变异为11.1783%~81.1753%;10个显性效应表现为显性正效应,LOD值在3.2787~163.7958之间。染色体2A的Br ID90127-Br ID10421区、Br ID10421-Br ID10709区及Br ID10709-Br ID101165区,5A的BRMS034(R5)-Ra3-H10区、6A的Ra1-F06-Ra2-D04区、8A的Br ID10839-Ra2-E12区和10A的Br ID90115-Ra2-E07区是多个性状QTL共享的标记区间。研究结果对冬油菜抗寒性基因精确定位具有一定价值。
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| 关 键 词： | 白菜型冬油菜 抗寒性 QTL 北方旱寒区 |
QTL analysis of cold tolerance for physiological traits of winter rapeseed in northern arid-cold area |
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| WU Jun-yan,KONG De-jing,FANG Yan,SUN Wan-cang,LIU Hai-qing,LIU Zi-gang,LI Xue-cai.QTL analysis of cold tolerance for physiological traits of winter rapeseed in northern arid-cold area[J].Agricultural Research in the Arid Areas,2017,35(1):205-210. |
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| Authors: | WU Jun-yan KONG De-jing FANG Yan SUN Wan-cang LIU Hai-qing LIU Zi-gang LI Xue-cai |
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| Institution: | College of Agronomy, Gansu Agricultural University, Gansu Key Lab of Crop Improvement and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou, Gansu 730070, China,College of Agronomy, Gansu Agricultural University, Gansu Key Lab of Crop Improvement and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou, Gansu 730070, China,College of Agronomy, Gansu Agricultural University, Gansu Key Lab of Crop Improvement and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou, Gansu 730070, China,College of Agronomy, Gansu Agricultural University, Gansu Key Lab of Crop Improvement and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou, Gansu 730070, China,College of Agronomy, Gansu Agricultural University, Gansu Key Lab of Crop Improvement and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou, Gansu 730070, China,College of Agronomy, Gansu Agricultural University, Gansu Key Lab of Crop Improvement and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou, Gansu 730070, China and College of Agronomy, Gansu Agricultural University, Gansu Key Lab of Crop Improvement and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou, Gansu 730070, China |
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| Abstract: | The superior cold resistance B .rapa variety “Longyou 7”and general cold variety “Longyou 9”were used as parents and a F2 mapping population was constructed .The results showed that 39 primers were selected for fur-ther analysis of F2 population .Based on molecular marker genetic map and QTL software,a total of 24 QTLs were detect-ed for SOD activity,POD activity,CAT activity,MDA content,free proline content and soluble protein content from 10 chromosomes .Those QTLs were able to explain 1 1 .1783%~81 .1753%of phenotypic variation .The dominant effects of 10 QTLs were positive effects,LOD values were between 3 .2787 ~163 .7958 .The data showed that the regions BrID90127-BrID10421,BrID10421-BrID10709 and BrID10709-BrID101165 on 2A,BRMS034(R5)-Ra3-H10 on 5A,Ra1-F06-Ra2-D04 on 6A,BrID10839-Ra2-E12 on 8A,BrID90115-Ra2-E07 on 10A were common regions shared by multi-traits .The result of present study is useful for the accurate position of winter rapa . |
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| Keywords: | winter rapeseed (Brassica rapa L ) cold resistance QTL northern arid-cold area |
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