低温胁迫下转ICE1基因水稻养分变化的研究

采用盆栽试验，选用转ICE1基因水稻T_4-8株系和T_4-9株系及未转基因水稻（Oryza sativa L.）品种垦鉴稻10号为试验材料，研究苗期低温胁迫对转基因水稻氮、磷、钾养分吸收的影响。结果表明，低温胁迫期间，转基因水稻幼苗丙二醛含量维持在较低水平，SOD活性维持在较高水平；地上部分氮、磷、钾含量变化不大，一直维持在较高水平；地下部分氮、磷、钾含量呈缓慢下降趋势，但降幅比非转基因水稻小，在胁迫第10天,T_4-8株系和T_4-9株系氮含量均高出对照13.86%，磷含量分别高出42.31%和50.00%，钾含量均高出85.71%。说明在低温胁迫条件下，ICE1基因过量表达可以增强水稻的抗寒性，减轻低温对水稻的伤害，保持根系较强的吸收养分能力。

Nutrient changes of transgenic ICE1 rice under low temperature stress

Using two transgenic ICE1 lines (T_4-8 and T_4-9) and normal cultivar (Kenjian 10) as test materials, the effects of low temperature stress on nutrient absorption of N, P and K in seedlings of rice (Oryza sativa L.) were investigated by pot experiment. The results showed that the MDA content kept at a low level while the SOD activity kept at a high level in transgenic rice seedlings. The contents of N, P and K in the above ground part varied little and kept at a high level. In the under-ground part, they showed a slow downward trend, but the decline rate in transgenic lines was less than that in normal cultivar. At the 10th day after low temper ature stress, the nitrogen content in under ground part of T_4-8 and T_4-9 was 13.86% higher, the phosphors content of T_4-8 and T_4-9 was 42.31% and 50.00% higher respectively, and the potassium content of T_4-8 and T_4-9 was 85.71% higher, compared with the control. In conclusion, over expression of ICE1 gene in rice could enhance cold tolerance, reduce the injury of rice plants and keep a strong absorption nutrient ability in roots under low temperature stress.