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| 苜蓿切叶蜂、金小蜂体内共生菌沃尔巴克氏体测定与分析 | |
| 引用本文： | 王翠敏,丛斌,米丰泉,戴秋慧,张兆琳.苜蓿切叶蜂、金小蜂体内共生菌沃尔巴克氏体测定与分析[J].中国植保导刊,2005,25(10):5-8. |
| 作者姓名： | 王翠敏 丛斌 米丰泉 戴秋慧 张兆琳 |
| 作者单位： | 1. 沈阳农业大学植物保护学院,辽宁,沈阳,110161;深圳出入境检验检疫局,广东,深圳,518045 2. 沈阳农业大学植物保护学院,辽宁,沈阳,110161 3. 辽宁出入境检验检疫局,辽宁,大连,116000 |
| 基金项目： | 国家十五科技攻关项目(2004BA509B04)，国家自然科学基金(30170625) |
| 摘 要： | 沃尔巴克氏体(Wolbachia)是广泛分布于节肢动物体内的一类共生菌,它们参与调控其寄主多种生殖活动的机制。本研究利用PCR方法对wsp基因的特异性扩增和测序,证实了Wolbachia在金小蜂体内的共生。并利用所测序列和其他已发表的序列建立系统树,为研究寄主与拟寄生物间的水平传播机制奠定了基础。 |
| 关 键 词： | 沃尔巴克氏体 苜蓿切叶蜂 金小蜂 wsp基因 水平传播机制 |
| 文章编号： | 1672-6820(2005)10-0005-04 |
| 修稿时间： | 2005年8月25日 |
Detection and analysis of Wolbachia endosymbiont in Megachile rotundata (F.) and Pteromalus venustus |
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| WANG Cui-min,CONG Bin,MI Feng-quan,DAI Qiu-hua,ZHANG Zhao-lin.Detection and analysis of Wolbachia endosymbiont in Megachile rotundata (F.) and Pteromalus venustus[J].China Plant Protection,2005,25(10):5-8. | |
| Authors: | WANG Cui-min CONG Bin MI Feng-quan DAI Qiu-hua ZHANG Zhao-lin |
| Institution: | WANG Cui-min1,2,CONG Bin1,MI Feng-quan1,DAI Qiu-hua1,ZHANG Zhao-lin1 |
| Abstract: | Wolbachia is a kind of symbioant bacteria commonly habiting in arthropod bodies, these bacteria participate in manipulating various reproducing mechanisms of their hosts# In this study, the presence of Wolbachia in Pteromalus venustus was detected based on amplification and sequencing the fragments of the wsp gene by PCR# Kinship relative tree was drawn by aligning the amplified fragment sequence with the published sequences, and the study provided the basis for further researching on horizontal transmission mechanism between host insects and parasitoids# |
| Keywords: | Wolbachia Megachile rotundata Pteromalus venustus wsp gene horizontal transmission mechanism |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |