水稻纹枯病菌强拮抗菌B34的分离与鉴定

 从水稻植株上分离到1株对水稻纹枯病菌有强烈拮抗作用编号为B34的菌株,在PDA平板上表现有对水稻纹枯病菌较强作用的抑菌带,其发酵菌液对纹枯病菌丝有强烈的致畸作用,抑菌率达到71.5%,使菌核的菌丝萌发率降低69.0%。离体防效和田间小区防效分别高达81%和48.74%,高于清水和井冈霉素处理。经生理生化检测及细胞壁化学成分分析,确定其为致黄假单胞菌(Pseudomonas aureofaciens)。

Screening, taxonomy of antagonistic strain B34 against Thanatephorus cucumeris

A strain B34 against Thanatephorus cucumeris was screened from rice plant. On the PDA plate a wide inhibition zone appeared. After using fermentation production treatment, the serious malformation effect was observed with electric microscope,the inhibition rate was 71.5%,and mycelial germination reduced rate of sclerotial was 69.0%. It showed that the control effect in vitro and in field reached 80% and 48.74% respectively,the result was higher than those of the water and Jinggangmycin treatments. From chemical components of cell wall, physiological and biochemical character of B34, the result showed it was Pseudomonas aureofaciens.