四川省100个小麦品种(系)抗条锈病基因的 分子检测

 为了解四川省近年小麦生产品种和后备品种对条锈菌致病类型G22-83和流行小种CYR32、CYR33的抗性水平,明确已知的主要抗条锈基因在该地区小麦品种中分布状况,利用小麦条锈菌G22-83、CYR32和CYR33对四川省100个小麦品种(系)进行苗期抗病性鉴定;采用已知基因Yr5、Yr9、Yr10、Yr15、Yr18 和Yr26的分子标记,对供试小麦材料进行抗性基因检测。结果表明,100个供试材料中,对CYR32 表现抗病的58份,占58%;对CYR33 表现抗病的63份,占63%;对G22表现抗病的43份,占43%;对CYR32、CYR33和G22均表现抗病性的品种(系)28个,占28%。供试小麦品种(系)中携带Yr9、Yr10、Yr15 和Yr26基因的材料分别有24份(24%)、9份(9%)、5份(5%)和26份(26%),所有供试品种(系)中未检测到Yr5和Yr18基因。

Molecular detection of stripe rust resistance gene(s) in 100 wheat cultivars (lines) from Sichuan Province in China

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is the most devastating wheat disease in China. Wheat growing region of Sichuan Province plays a key role for stripe rust spread and epidemic in China. To evaluate the resistant level and detect the stripe rust resistance gene(s) of commercial wheat cultivars and advanced lines in Sichuan Province, 100 wheat cultivars (lines) collected from Sichuan Province were inoculated with Chinese Pst races G22-83, CYR32 and CYR33 at the seedling stage, and the genomic DNA of these cultivars (lines) were tested with the closely linked markers of all-stage resistance genes Yr5, Yr9, Yr10, Yr15, Yr26 and adult-plant resistance gene Yr18. The disease evaluation results indicated that 58%, 63% and 43% wheat cultivars (lines) showed resistance to CYR32, CYR33 and G22-83 in the seedling tests, respectively. Among these, 28 cultivars (lines) were resistant to all three Pst races. Molecular detection suggested that the resistance genes Yr9, Yr10, Yr15 and Yr26 were found in 24%, 9%, 5% and 26% cultivars (lines), respectively. There was no indication of Yr5 and Yr18 in any cultivar (lines).