假禾谷镰孢菌细胞自噬相关基因的鉴定与表达分析

细胞自噬是真核生物中一类包含多种进化保守的Atgs蛋白参与的蛋白质降解途径,该途径也参与多种植物病原真菌的产孢、孢子萌发和致病性过程。本研究利用模式物种已知的Atgs,通过生物信息学分析在假禾谷镰孢菌中鉴定到29个Atgs,分属29种不同蛋白类型。细胞自噬核心调控机制相关蛋白Atg1、Atg6、Atg8和Atg9的氨基酸序列系统进化分析结果显示,Atgs在丝状真菌间非常保守,符合物种进化的特点。通过假禾谷镰孢菌跟亲和与非亲和寄主转录组数据分析发现,与菌丝阶段相比26个Fp Atgs在侵染阶段的表达发生改变,其中Fp Atg1、2、3、4、5、7、8、9、13、22、23、28和Fp Vps34在侵染初期和中后期均诱导表达;Fp Atg6、14、17、20、24、29和Fp Vps15在侵染初期和中后期表达量均降低;Fp Atg10、11除在非亲和互作中后期表达量略有上调,其余阶段均显著下调表达;Fp Atg15、16在亲和互作初期诱导表达,其余阶段均下调表达;Fp Atg26、27在亲和互作初期下调表达,中后期诱导表达,而在非亲和互作初期表达量升高,到中后期下调表达。推测细胞自噬参与调控假禾谷镰孢菌的侵染过程。

Identification and expression analysis of autophagy-related gene in Fusarium pseudograminearum

Autophagy, regulated by a large set of Atgs, is a conserved mechanism for protein degradation in eukaryotes with roles in sporulation, germination and the virulence of plant fungal pathogens. In this study, we identified 29 autophagy-related proteins (Atgs) belonging to 29 different groups in Fusarium pseudograminearum using a genome-wide survey; Atgs seem to be highly conserved compared with filamentous fungi by the phylogenetic trees of Atg1, Atg6, Atg8 and Atg9. Expression profile analysis of the F. pseudograminearum affinity interaction and the non-affinity interaction revealed that FpAtgs are broadly expressed and that 26 of them show differential expression during infection stages compared with in mycelia. FpAtgs, including FpAtg1, 2, 3, 4, 5, 7, 8, 9, 13, 22, 23, 28 and FpVps34, show a relatively higher expression level during early and later infection stages, while FpAtg6, 14, 17, 20, 24, 29 and FpVps15 exhibited a low expression level. In addition, FpAtg10, 11 showed slightly up-regulation expression in later stage of the non-affinity interaction, and down-regulation expression in other infection stages. FpAtg15,16 are induced in early stage of the affinity interaction, and down-regulation expression in other infection stages. FpAtg26 and 27 show down-regulation expression in early stage of the affinity interaction, and up-regulation expression in the later. However, these two genes showed opposite expression patterns in the non-affinity interaction. These results suggested that autophagy might play roles in infection mechanism of F. pseudograminearum.