| 进境澳大利亚豌豆细菌性萎蔫病病原菌的分离鉴定 |
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| 引用本文： | 崔汝强,胡学难,廖金铃,钟国强,郭权,王卫芳,冯黎霞,赵立荣,吴海荣,胡佳.进境澳大利亚豌豆细菌性萎蔫病病原菌的分离鉴定[J].植物病理学报,2009,39(3):238-242. |
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| 作者姓名： | 崔汝强 胡学难 廖金铃 钟国强 郭权 王卫芳 冯黎霞 赵立荣 吴海荣 胡佳 |
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| 作者单位： | 1 广东出入境检验检疫局, 广州 510623;2 华南农业大学植物线虫研究室, 广州 510642 |
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| 摘 要： | 2008年3月自澳大利亚进口的豌豆种苗病变部位分离到致病性菌株,该菌株能引起豌豆茎部、卷须萎蔫腐烂,叶片褪绿等症状。针刺接种豌豆,发生的病变状态与自然发病的症状相同,且在接种发病植株中又分离到形态上一致的病原菌,得到了柯赫法则的验证。经形态特征、培养性状、生理生化及16S rDNA序列分析,鉴定其为桃色欧文氏菌(Erwinia persicina)。这是我国首次从进境豌豆中检出该病害。
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| 关 键 词： | 豌豆 细菌性病害 病原鉴定 桃色欧文氏菌 |
Isolation and identification of bacterial pathogen causing wilt on pea seed imported from Australia |
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| CUI Ru-qiang,HU Xue-nan,LIAO Jin-ling,ZHONG Guo-qiang,GUO Quan,WANG Wei-fang,FENG Li-xia,ZHAO Li-rong,WU Hai-rong,HU Jia.Isolation and identification of bacterial pathogen causing wilt on pea seed imported from Australia[J].Acta Phytopathologica Sinica,2009,39(3):238-242. |
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| Authors: | CUI Ru-qiang HU Xue-nan LIAO Jin-ling ZHONG Guo-qiang GUO Quan WANG Wei-fang FENG Li-xia ZHAO Li-rong WU Hai-rong HU Jia |
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| Institution: | 1 Guangdong Exit-Entry Inspection and Quarantine Bureau, Guangzhou 510623, China;2 The Laboratory of Plant Nematode, South China Agricultural University, Guangzhou 510642, China |
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| Abstract: | Three isolates were isolated from diseased seedling stems of pea imported from Australia in March 2008.Inoculation on pea seedlings with these isolates caused the same symptoms as naturally infected plants,while symptoms did not occur on control plants(inoculated with sterile water).The bacteria sharing the cha-racteristics of the inoculated isolates were recovered from symptomatic plants,hence fulfilling Koch’s postulates.All three isolates were identified as Erwinia persicina by pathogenicity,staining reactions,morphological characteristics,culture patterns,physiological and biochemical reactions,and 16S rDNA gene sequences. |
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| Keywords: | pea bacterial disease pathogen identification Erwinia persicina |
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