蚕豆萎蔫病毒2号分离物PV131和P158侵染寄主植物的细胞病理比较观察

 透射电镜观察比较了蚕豆萎蔫病毒2号(BBWV-2)分离物PV131和P158侵染昆诺藜(Chenopodium quinoa)和蚕豆(Vicia faba)的细胞超微结构变化。结果显示:受PV131分离物侵染的昆诺藜叶肉细胞质中膜结构大量增生,形成有膜状结构、大小泡囊及电子致密物质的特殊膜增生区;病毒粒子散布在细胞质中,形成大块结晶体和管状结构,该管状结构直径约75nm,横切面由9个病毒粒子组成;受PV131侵染的蚕豆叶肉细胞中也形成膜增生区和管状结构,未见病毒结晶体。受P158分离物侵染的昆诺藜和蚕豆叶肉细胞病变情况与PV131分离物相似,形成膜增生区和相同的管状结构,在蚕豆叶肉细胞中也未观察到病毒结晶体。上述结果表明:2个BBWV-2分离物虽然在不同寄主植物上引起的细胞病变程度有差异,但其细胞病理学特征是由病毒基因结构所决定,与寄主的种类无关。

Comparison of cytopathological changes of plant hosts infected with two Broad bean wilt virus 2 isolates PV131 and P158

Both the Chenopodium quinoa and Vicia faba were respectively infected with two Broad bean wilt virus 2 (BBWV-2) isolates, PV131 and P158, and their cytopathological changes were observed by transmission electron microscope. In the PV131 isolate infected C. quinoa cells, the membrane proliferated and contained vesicles and electron-dense materials. The extensive proliferation of membrane might act as the viral replication center. Viral particles distributed in the cytoplasm and some of them accumulated into crystals or aggregated into tubules. The diameter of the tubule was about 75 nm and there were nine viral particles on the transverses section. The similar data were got from the other three kinds of infected mesophyll cells but PV131 and P158 virions did not accumulate into crystals in V. faba cells. All the observations indicated that the differences of pathogenicity between PV131 and P158 isolate were determined by their genome but not the host types.