一个小麦茉莉酮酸酯诱导蛋白基因的克隆和鉴定

 用基因芯片技术结合分池法(bulked segregating analysis,BSA)对参与小麦(Triticum aestivum L.)"兰考90(6)"抗白粉病反应或与抗病基因连锁的EST进行了分析。从"中国春"中克隆了一个与Ta-JA1高度相似的新的小麦茉莉酮酸酯诱导蛋白基因(GenBank登录号:EU035635),命名为Ta-JA2。Ta-JA2和Ta-JA1的cDNA序列有99%相同,编码304个氨基酸组成的多肽。Ta-JA2具有植物病原应答诱导蛋白-dirigent-类蛋白的典型保守功能域和jacalin-类植物血球凝集素的典型保守功能域。Ta-JA2主要在叶片和茎中表达,在根和幼穗中几乎不表达;在幼叶、壮叶和旗叶中的表达水平依次增强;在"中国春"和一个二粒小麦(Triticum dicoccoides)品系幼叶中的表达受白粉菌(Blumeria graminis f.sp.tritici)的诱导而增强;在"兰考90(6)21-12"叶片中的表达保持稳定而较高的水平。根据氨基酸序列的相似性建立了Ta-JA2类似蛋白的树形图,提供了植物中此类基因的进化信息。

Cloning and characterization of a jasmonate-induced protein gene in wheat

Gene chip hybridization combined with bulked segregating analysis(BSA) was used to identify ESTs involved in powdery mildew resistance or linked to the resistance gene in wheat(Triticum aestivum L.) line ‘Lankao 90(6)’.A new jacalin gene(GenBank accession number:EU035635) highly similar to Ta-JA1 was isolated from ‘Chinese Spring’,and was designated Ta-JA2.cDNA sequences of Ta-JA1 and Ta-JA2 showed 99% identity.Ta-JA2 encoded a 304 amino acid constituted peptide.Ta-JA2 protein had a typical domain for plant disease response dirigent-like proteins and a typical domain for jacalin-like lectins.Ta-JA2 expressed in leaves and stems,but almost not in roots and young spikes.The expression level of Ta-JA2 was increased in turn of young leaves,strong leaves and flag leaves.The transcribing was enhanced in seedling leaves of ‘Chinese Spring’ and a Triticum dicoccoides line by Blumeria graminis f.sp.tritici.The expression level of Ta-JA2 in ‘Lankao 90(6) 21-12’ seedling leaves was stable and relatively higher.A rectangle tree of Ta-JA2 and the similar proteins was constructed based on sequence similarity.The study provided some evolution information of the jacalin-like proteins in plants.