偃麦草属E染色体组特异SCAR标记对Lr19的特异性和稳定性研究

 小麦抗叶锈基因Lr19来源于长穗偃麦草,表现优良的抗叶锈性,国内外发现对Lr19有毒性的小麦叶锈菌菌株的报道较少。本研究以TcLr19和Thatcher亲本以及TcLr19×Thatcher F₂代单株构建的分离群体为材料,建立了与Lr19共分离的稳定的SCAR分子标记,命名为Y₁₉SCAR₉₈₂。对49个小麦抗叶锈近等基因系材料的稳定性检测结果表明,其重复性好且为Lr19特异的分子标记。对120个小麦品种检测的结果表明,该标记可有效应用于小麦抗叶锈分子辅助选择育种。

Specificity and stability of E-chromosome specific SCAR marker from Thinopyrum spp.for Lr19

Leaf rust resistance gene Lr19 transferred from Thinopyrum elongatum into wheat cultivars has expressed resistance to most pathotypes of Puccinia triticina in China. To identify the molecular marker linked to Lr19, a segregating F₂ population from a cross between the resistance parent TcLr19 and susceptible parent Thatcher were screened. A SCAR marker cosegregating with Lr19 was found and named as Y₁₉SCAR₉₈₂. The result of stability validation test on the 49 international set of near-isogenic lines in Thatcher background suggested that Y₁₉SCAR₉₈₂ was specific and sufficient for Lr19. The detection of the marker in 120 wheat varieties indicated that it could be effectively used for MAS in breeding of the resistance to leaf rust in wheat.