抗白粉病小麦-中间偃麦草双体异附加系的鉴定

 对从中间偃麦草与普通小麦品种烟农15杂交后代(BC₃F₆)中选育的双体异附加系山农Line15的形态学、白粉病抗性、细胞学、基因组荧光原位杂交(GISH)及RAPD进行鉴定分析.结果表明它的主要形态性状介于双亲之间;白粉病抗性鉴定结果表明山农Line15对白粉病高抗近免疫;根尖细胞染色体数目为2n=44,PMC MI染色体构型为2n=22Ⅱ;以中间偃麦草总基因组DNA为探针的基因组荧光原位杂交(GISH),结果表明山农Line15是在小麦的遗传背景中附加了2条中间偃麦草染色体,为小麦-中间偃麦草双体异附加系;遗传分析表明山农Line15的抗白粉病基因基本上可以确定来源于中间偃麦草的染色体;RAPD分析表明:在供试的120个随机引物中有1个引物S170(-5'-ACA ACG CGA G-3'-)能在山农Line15中稳定地扩增出特异带型,可以作为山农Line15所附加的中间偃麦草染色体的特异分子标记.

Identification of wheat-Elytrigia intermedium alien disomic addition line with powdery mildew resistance

An alien disomic addition line,Shannong Line15,which derived from the progeny of Elytrigia intermedium and common wheat Yannong 15(BC₃F₆),was identified by morphology,powdery mildew resis-tance,cytology,genome in situ hybridization (GISH)and random amplified polymorphic DNA (RAPD)analysis.The results demonstrated that Shannong Line15,with the chromosome number of 2n=44 in root tip cells and chromosome configuration of 2n=22Ⅱat PMC MⅠ,was highly resistant or immune to powdery mildew and its main morphological traits were between that of their parents. The results of GISH with the probe of total genomic DNA of E. intermedium further proved that Shannong Line15 was an alien disomic addition line with a pair of E.intermedium chromosomes in wheat background.Genetic analysis showed that powdery mildew resistant genes of Shannong Line15 most probably came from the chromosomes of E.intermedium. Among 120 RAPD primers,the primer S170 (-5'-ACA ACG CGA G-3'-) could amplify specific bands of E.intermedium which could be used as RAPD markers for additional E.intermedium chromosomes of Shannong Line15.