| 瞬时表达比较2种不同的RNAi载体的干涉效果 |
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| 引用本文： | 邱礽,陶刚,邱又彬,李奇科,朱英,迟胜起,刘作易.瞬时表达比较2种不同的RNAi载体的干涉效果[J].植物病理学报,2010,40(5):543-547. |
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| 作者姓名： | 邱礽 陶刚 邱又彬 李奇科 朱英 迟胜起 刘作易 |
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| 作者单位： | 1 贵州大学生命科学院, 贵阳 550025;2 贵州省农业生物技术重点实验室, 贵阳 550006;3 贵州省植物保护研究所, 贵阳 550006;4 贵州省生物技术研究所, 贵阳 550006;5 青岛农业大学植物保护学院, 青岛 266109 |
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| 基金项目： | "973"计划前期研究专项，贵州省科学技术重大专项，贵州省农业科学院重点专项项目 |
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| 摘 要： | RNAi with natural defence mechanism of homologous RNA degradation is widely used in research of antiviral plant. It is important to construct a highly efficent RNAi vector for transgenic plants of virus resis-tance. In this study, part fragments of coat protein gene of Potato virus Y (PVY) (451-750 bp) were inserted into the two expression vectors. Vector pROKY300 without intron and pHelY300 with PDK and CAT introns on the hpRNA stem were constructed. The silence efficiency of virus resistance of the two vectors was investigated as 88% (22/25)for pROKY300 and 92% (23/25) for pHelY300 through transient expression mediated by agroinfiltration. The results showed that both vectors were highly antiviral and elucidated the validity of RNAi-medicates resistance to virus.
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Comparision of resistance to Potato virus Y(PVY) of two different RNAi vectors via transient expression |
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| QIU Reng,TAO Gang,QIU You-bin,LI Qi-ke,ZHU Ying,CHI Sheng-qi,LIU Zuo-yi.Comparision of resistance to Potato virus Y(PVY) of two different RNAi vectors via transient expression[J].Acta Phytopathologica Sinica,2010,40(5):543-547. |
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| Authors: | QIU Reng TAO Gang QIU You-bin LI Qi-ke ZHU Ying CHI Sheng-qi LIU Zuo-yi |
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| Institution: | 1 College of Life Science, Guizhou University, Guiyang 550025, China;2 The Key Laboratory for Agricultural Biotechnology of Guizhou, Guiyang 550006, China;3 Guizhou Institute of Plant Protection, Guiyang 550006, China;4 Guizhou Institute of Biotechnology, Guiyang 550006, China;5 Plant Protection College, Qingdao Agricultural University, Qingdao 266109, China |
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| Abstract: | RNAi with natural defence mechanism of homologous RNA degradation is widely used in research of antiviral plant. It is important to construct a highly efficent RNAi vector for transgenic plants of virus resis-tance. In this study, part fragments of coat protein gene of Potato virus Y (PVY) (451-750 bp) were inserted into the two expression vectors. Vector pROKY300 without intron and pHelY300 with PDK and CAT introns on the hpRNA stem were constructed. The silence efficiency of virus resistance of the two vectors was investigated as 88% (22/25)for pROKY300 and 92% (23/25) for pHelY300 through transient expression mediated by agroinfiltration. The results showed that both vectors were highly antiviral and elucidated the validity of RNAi-medicates resistance to virus. |
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| Keywords: | RNAi agroinfiltration transient expression PVY CP |
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