小麦白粉病菌有性生殖与自然群体交配型检测

本研究根据MAT1-1-1(Gen Bank登录号HQ171902)和MAT1-2-1(Gen Bank登录号HQ171899)的部分基因序列设计和筛选其特异性引物,使用PCR方法对2012年度采自四川、陕西、云南、贵州、河南、安徽、江苏、河北、山东和甘肃10个省30个采集地点共516个小麦白粉病菌菌株的交配型基因进行检测。结果显示30个采集地点两种交配型基因都能检测到,其中28个采集地的两种交配型菌株比例(MAT1-1-1∶MAT1-2-1)符合1∶1。选择具不同交配型基因的小麦白粉病菌株配对接种,16个随机杂交组合都能产生闭囊壳。对获得的闭囊壳进行子囊孢子释放,均可获得子囊孢子。该研究结果为深入研究有性生殖对小麦白粉病菌株毒性多样性的作用奠定了基础。

Sexual reproduction and detection of mating-type of Blumeria graminis f. sp. tritici populations

Pairs of primers were designed according to the partial sequences of MAT1-1-1 (GenBank accession No.: HQ171902) MAT1-2-1 (GenBank accession No.: HQ171899) genes of Blumeria graminis. f. sp. tritici and screened specific primer sets for detection of the two mating-type genes. Based on PCR amplification, 516 of single ascospore isolates established from thirty counties of 10 provinces were employed to detect mating-type genes in this study. The results indicated that the two mating type genes were detected in all of isolates from each of 30 counties, and isolates collected from 28 counties were fit to a ratio of nearly 1:1 (MAT1-1-1 : MAT1-2-1). 16 crosses between isolates of different mating-type were made by pairing randomly and then transferring one isolates to another on plants. Successful formations of cleistothecia after crosses were observed in pairs of the sixteen crosses. Ascospores can release from cleistothecia produced form all of the sixteen crosses. These results in this study provide a basis for further studying role of sexual reproduction in virulence diversity of B. graminis f. sp. tritici.