| 南疆红枣黑斑病病原鉴定及其保守基因序列分析 |
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| 引用本文： | 宋博,徐兵强,朱晓锋,阿布都克尤木·卡德尔,杨森.南疆红枣黑斑病病原鉴定及其保守基因序列分析[J].植物病理学报,2017,47(3):411-415. |
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| 作者姓名： | 宋博 徐兵强 朱晓锋 阿布都克尤木·卡德尔 杨森 |
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| 作者单位： | 新疆农业科学院植物保护研究所,特色林果产业国家地方联合工程研究中心,农业部西北荒漠作物有害生物综合治理重点实验室,乌鲁木齐 830091 |
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| 基金项目： | 自治区财政林业科技专项资金项目(新林计字[2013]45号);自治区科技计划项目(201130102-3) |
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| 摘 要： | 正红枣黑斑病(jujube black spot)又称"烂果病"、"黑头病",在新疆南疆地区发生严重。2013—2014年阿克苏、和田地区红枣黑斑病发病率达20%~30%,重病枣园发病率高达40%~50%。红枣黑斑病严重制约着新疆红枣产业的健康发展。目前,国内在红枣黑斑病病原认知上仍存在较大分歧,报道的病原主要为链格孢(Alternaria alternata)~(1~3]),也有细极链格孢(Alternaria tenuissima)危害的报道~(4])。本文通过对南疆红枣黑斑病菌形态特征研究和田间病原回接试验,结合分子生物学手
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Identification of jujube black spot pathogens in Southern Xinjiang and its sequence analysis of conserved genes |
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| SONG Bo,XU Bing-qiang,ZHU Xiao-feng,Abudu Keyimu·Kader,YANG Sen.Identification of jujube black spot pathogens in Southern Xinjiang and its sequence analysis of conserved genes[J].Acta Phytopathologica Sinica,2017,47(3):411-415. |
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| Authors: | SONG Bo XU Bing-qiang ZHU Xiao-feng Abudu Keyimu·Kader YANG Sen |
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| Institution: | Institute of Plant Protection, Xinjiang Academy of Agricultural Science, National Local Joint Engineering Research Center of Special Forestry and Fruit Industry, Key Laboratory of Integrated Pest Management on Crops in Northwestern Oasis Ministry of Agriculture, Urumqi 830091, China |
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| Abstract: | A study was conducted to identify the pathogen of jujube black spot. The isolates were obtained from diseased jujube tissues with typical black spot symptoms by conventional tissue isolation method. The pathogenicity test was conducted and fulfilled by Koch′s postulates. The pathogen was identified based on morphology characters and analysis of PCR amplification by the general primers of fungal conserved genes (ITS, GAPDH and EF-1α). All sequences were deposited into the NCBI database with the GenBank accession number: KU145270, KU145272 and KU145274. Taken together, we concluded that Alternaria tenuissima is the causing agent for the jujube black spot disease. |
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| Keywords: | black spot Alternaria tenuissima ITS GAPDH EF-1α |
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