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野生番茄花叶病毒在四川侵染烟草的简报
引用本文:孙航军,杨金广,李锡宏,余广宏,孙冬梅,王凤龙.野生番茄花叶病毒在四川侵染烟草的简报[J].植物病理学报,2015,45(6):680-684.
作者姓名:孙航军  杨金广  李锡宏  余广宏  孙冬梅  王凤龙
作者单位:黑龙江八一农垦大学,大庆 163319;
中国农业科学院烟草研究所,青岛 266101;
湖北省烟草科研所,武汉 430030;
云南烟草保山香料烟有限责任公司,保山 678000
基金项目:云南省烟草公司科技项目(2013YN37);湖北省烟草公司科技项目(027Y2013-006);四川省烟草专卖局项目(201101007)
摘    要:<正>在植物病毒中,马铃薯Y病毒属(Potyvirus是最大的正链RNA病毒属,是已确定的植物病毒属中成员最多的一个。Potyvirus基因组长10 000nt左右,3'末端有多聚腺苷酸尾,编码一个大的多聚蛋白,可被剪切为10个功能蛋白。病毒粒体长700~900 nm,直径11~13 nm,经蚜虫以非持久方式传播,可侵染超过500种植物,包括茄科、苋科、


Brief Report of a Wild tomato mosaic virus isolate infecting Nicotiana tabacum in Sichuan
SUN Hang-jun,YANG Jin-guang,LI Xi-hong,YU Guang-hong,SUN Dong-mei,WANG Feng-long.Brief Report of a Wild tomato mosaic virus isolate infecting Nicotiana tabacum in Sichuan[J].Acta Phytopathologica Sinica,2015,45(6):680-684.
Authors:SUN Hang-jun  YANG Jin-guang  LI Xi-hong  YU Guang-hong  SUN Dong-mei  WANG Feng-long
Institution:Heilongjiang Bayi Agricultural University, Daqing 163319, China;
Tobaco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China;
Hubei Tobacco Science Institute, Wuhan 430030, China;
Yunnan Baoshan Oriental Tobacco Corporation, Baoshan 678000, China
Abstract:XC-1, a virus isolate, was identified in Nicotiana tabacum showing severe mosaic symptom in Xichang, Sichuan province of China, electron microscopy of the XC-1 revealed that it had flexuous filamentous particles with a diameter of 13 nm and length about 740-760 nm, N. tabacum NC89 inoculated with XC-1 developed vein clearing 5 days post-inoculation (dpi) and presented mosaic symptom 8-14 dpi. 3′terminal sequence of XC-1 was obtained by RT-PCR, XC-1 was clustered with other Wild tomato mosaic virus (WTMV) isolates in phylogenetic tree based on the CP gene sequence, and shared the highest identity (94.34%) with WTMV isolate from Guangdong by homologous alignment and cluster analysis, indicating that XC-1 was a WTMV isolate named WTMV-XC-1.
Keywords:Wild tomato mosaic virus  Nicotiana tabacum  Sichuan  identification  
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