青枯雷尔氏菌胞外蛋白指纹多态性分析

 采用SDS-PAGE技术对70株不同来源及致病力青枯雷尔氏菌（Ralstonia solanacearum）进行胞外蛋白指纹多态性分析，研究结果表明，供试青枯雷尔氏菌菌株呈现丰富的胞外蛋白指纹多态性，多态性比率为100%。不同来源青枯雷尔氏菌分泌的胞外蛋白不同，EZ-Tn5TM插入诱变菌株电泳出20条不同大小蛋白条带，分子量集中在20~97 kD ，且菌株间蛋白条带相似或完全相同；60Co辐射诱变菌株共电泳出16条不同大小的蛋白条带，多数蛋白分子量44.3 kD；野生型菌株电泳的蛋白条带最多，共获得26条不同大小的蛋白条带。进一步对37株不同致病力的野生型菌株进行胞外蛋白含量测定，结果表明，不同致病力菌株胞外蛋白含量差异大，强致病力菌株分泌的胞外蛋白含量较高，为1.026～5.963μg/mL，无致病力菌株胞外蛋白含量较低，为0.083～0.761 μg/mL。

Polymorphism analysis of extracellular protein from Ralstonia solanacearum

By using SDS-PAGE, the extracellular protein polymorphism of 70 Ralstonia solanacearum wild-type and mutant strains were analyzed. The tested strains possessed abundant variation highly associated with their origins. Twenty protein bands were detected in the EZ-Tn5 TM transponson-generated mutants with a range molecular weights from 20-97 kD, and all mutants shared a highly similar protein band profile. The strains from 60Co-radiated mutagenesis produced 16 protein bands, most of which had molecular weight lower than 44.3 kD. The wild-type strains isolated from diseased plant samples produced the most abundant extracellular proteins (26 bands). Furthermore, extracellular protein concentrations of 37 wild-type R. solanacearum strains with different pathogenicity were determined. The virulent strains produced higher contents of extracellular proteins (1.026 to 5.963μg/mL) than that of avirulent strains (0.083 to 0.761 μg/mL).