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小麦品种(系)抗白粉病基因推导及分子标记鉴定
引用本文:高海峰,范洁茹,周益林,王锁牢,白微微,李广阔.小麦品种(系)抗白粉病基因推导及分子标记鉴定[J].植物病理学报,2017,47(3):370-379.
作者姓名:高海峰  范洁茹  周益林  王锁牢  白微微  李广阔
作者单位:新疆农业科学院植物保护研究所/农业部西北荒漠绿洲作物有害生物综合治理重点实验室,乌鲁木齐 830091;
中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室,北京 100193
基金项目:新疆维吾尔自治区科技支疆项目(2013911092);公益性行业(农业)科研专项(201303016);农业部西北荒漠绿洲作物有害生物综合治理重点实验室开放基金(KFJJ20160106);新疆农科院青年科技基金项目(xjnky-2014005);新疆维吾尔自治区青年科学基金(2016D01B045)
摘    要:利用基因推导法和分子标记对我国主要麦区的小麦品种(系)进行了抗白粉病基因的鉴定。结果表明,南30-10等15个品种(系)含有Pm8,新麦2号等9个品种(系)含有Pm4,中植4号等9个品种(系)含有Pm21,郑麦113含有Pm4b+5b,杨09-111和新紫1号含有Pm2+mld。研究发现,基因推导和分子标记相结合,可大大提高小麦品种(系)抗白粉病基因鉴定结果的准确性,鉴定结果可为抗病育种和品种布局及白粉病的防治提供依据。

关 键 词:小麦    白粉病  抗病基因  基因推导  分子标记  

Postulation and molecular marker detection of powdery mildew resistance genes in wheat cultivars (lines)
GAO Hai-feng,FAN Jie-ru,ZHOU Yi-lin,WANG Suo-lao,BAI Wei-wei,LI Guang-kuo.Postulation and molecular marker detection of powdery mildew resistance genes in wheat cultivars (lines)[J].Acta Phytopathologica Sinica,2017,47(3):370-379.
Authors:GAO Hai-feng  FAN Jie-ru  ZHOU Yi-lin  WANG Suo-lao  BAI Wei-wei  LI Guang-kuo
Institution:Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences / Key Laboratory of Integrated Pest Management on Crop in Northwestern Oasis, Ministry of Agriculture, P. R. China, Urumqi 830000, China;
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Abstract:Powdery mildew resistance genes in wheat cultivars (lines) from major Chinese wheat regions were studied by using gene postulation and molecular markers. The results indicated that Pm8 was the most postulated or detected genes carried by fifteen cultivars (lines). Pm4 presented in nine cultivars (lines). Pm21 presented in nine cultivars (lines). Zhengmai113 was postulated to possess Pm4b+5b. Yang 09-111 and Xinzi No.1 was postulated to possess Pm2+mld . The results showed that the combination of gene postulation and molecular markers can greatly improve the accuracy of the identification to powdery mildew resistance gene in wheat cultivars (lines). The identification results can provide the basis for disease resistance breeding, cultivar deployment the prevention and control of powdery mildew.
Keywords:wheat  powdery mildew  resistance gene  gene postulation  molecular marker  
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