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云南、福建、湖南烟区烟草花叶病主要病毒种类检测及黄瓜花叶病毒亚组鉴定
引用本文:刘勇,莫笑晗,余清,顾钢,黄石旺,夏玉珍,周雪平.云南、福建、湖南烟区烟草花叶病主要病毒种类检测及黄瓜花叶病毒亚组鉴定[J].植物病理学报,2006,36(4):310-313.
作者姓名:刘勇  莫笑晗  余清  顾钢  黄石旺  夏玉珍  周雪平
作者单位:1 浙江大学生物技术研究所, 杭州 310029;2 云南省烟草科学研究所, 玉溪 653100;3 福建省烟草农业科学研究所, 福州 350003;4 中国烟草中南实验站永州基地, 永州 425000
基金项目:国家烟草专卖局资助项目;云南省烟草公司资助项目
摘    要: 采用抗原直接包被和双抗体夹心酶联免疫吸附测定法(ELISA)对采自云南、福建、湖南烟区烟草花叶病样品进行了病毒种类检测,利用三抗体夹心ELISA对黄瓜花叶病毒(Cucumber mosaic virus,CMV)的亚组类型进行了鉴定。在云南采集的520个花叶病样品中,烟草花叶病毒(Tobacco mosaic virus,TMV)、CMV和马铃薯Y病毒(Potato virus Y,PVY)总检出率分别为71.74%、55.01%和6.35%;在福建采集的150个花叶病样品中,TMV、CMV和PVY的总检出率分别为94%、24.66%和8.00%;在湖南采集的74个花叶病样品中,TMV、CMV和PVY的总检出率分别为58.11%、51.35%和2.70%。部分样品为2种以上病毒复合侵染。云南、福建和湖南采集的64个CMV阳性样品中,属亚组Ⅰ的样品为57个,占89.1%;属亚组Ⅱ的样品为10个,占15.6%;其中3个样品为亚组Ⅰ和亚组Ⅱ的复合侵染。

关 键 词:烟草  黄瓜花叶病毒  烟草花叶病毒  马铃薯Y病毒  检测  亚组分类  
文章编号:0412-0914(2006)04-0310-04
修稿时间:2005年9月5日

Virus detection of tobacco mosaic disease and subgrouping of Cucumber mosaic virus (CMV) in Yunnan, Fujian and Hunan Provinces
LIU Yong,MO Xiao-han,YU Qing,GU Gang,HUANG Shi-wang,XIA Yu-zhen,ZHOU Xue-ping.Virus detection of tobacco mosaic disease and subgrouping of Cucumber mosaic virus (CMV) in Yunnan, Fujian and Hunan Provinces[J].Acta Phytopathologica Sinica,2006,36(4):310-313.
Authors:LIU Yong  MO Xiao-han  YU Qing  GU Gang  HUANG Shi-wang  XIA Yu-zhen  ZHOU Xue-ping
Institution:1 Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China;2 Yunnan Institute of Tobacco Science, Yuxi 653100, China;3 Fujian Provincial Institute of Tobacco Science, Fuzhou 350003, China;4 Yongzhou Base, Zhongnan Experiment Station, Yongzhou 425000, China
Abstract:Tobacco leaf samples with mosaic symptoms collected from Yunnan, Fujian and Hunan Provinces were tested for virus types by using antigen direct coated or double antibody sandwich enzyme-linked immunosorbent assay(ELISA), and triple antibody sandwich(TAS) ELISA was then used for subgrouping of Cucumber mosaic virus(CMV) samples. Among 520 tobacco samples collected in Yunnan Province, 71.74%, 55.01% and 6.35% samples were infected by Tobacco mosaic virus(TMV), CMV and Potato virus Y(PVY), respectively. Among 150 tobacco samples collected in Fujian Province, 94%, 24.66% and 8.00% samples were infected by TMV, CMV and PVY, respectively. Among 74 tobacco samples collected in Hunan Province, 58.11%, 51.35% and 2.70% samples were infected by TMV, CMV and PVY, respectively. Some samples were infected with more than two viruses. Among 64 CMV samples, 89.1% samples were infected with subgroupⅠisolates and 15.6% were infected with subgroupⅡ isolates, three samples were infected with both subgroupⅠand subgroupⅡ isolates.
Keywords:tobacco  Cucumber mosaic virus  Tobacco mosaic virus  Potato virus Y  detection  subgroup  
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