| 一个二粒小麦LRR类受体蛋白激酶基因的克隆和鉴定 |
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| 引用本文： | 牛吉山,常阳,刘瑞,倪永静.一个二粒小麦LRR类受体蛋白激酶基因的克隆和鉴定[J].植物病理学报,2009,39(2):217-220. |
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| 作者姓名： | 牛吉山 常阳 刘瑞 倪永静 |
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| 作者单位： | 河南农业大学国家小麦工程技术研究中心, 郑州 450002 |
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| 基金项目： | 河南省杰出科研人才创新工程项目 |
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| 摘 要： | Near the HMW-glutenin gene of wheat (Triticum aestivum), there is a locus (temporarily named TaXa) encoding LRR-receptor-like protein kinase, which is homologous to disease resistance protein Xa21 of rice (Oryza sativa). Through RT-PCR approach, a cDNA clone of ZS2002 was isolated from the orthologous locus of TaXa in Triticum turgidum. ZS2002 was 3 081 bp long and encoding a peptide composed of 1 026 amino acid. The protein included N-terminal conserved sequence, LRR domains, a transmembrane region and a serine/threonine protein kinase domain. ZS2002 was expressed in root, stem, leaf and spike. The transcribing in seedling leaves was significantly enhanced by Blumeria graminis f.sp. tritici. TaXa gene might play a role in powdery mildew resistance reaction in Triticum.
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| 关 键 词： | 类受体蛋白激酶 蛋白激酶基因 二粒小麦 胞外结构域 鉴定 克隆 R类 信号传导途径 |
Cloning and characterization of a LRR-receptor-like protein kinase gene from Triticum turgidum |
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| NIU Ji-shan,CHANG Yang,LIU Rui,NI Yong-jing.Cloning and characterization of a LRR-receptor-like protein kinase gene from Triticum turgidum[J].Acta Phytopathologica Sinica,2009,39(2):217-220. |
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| Authors: | NIU Ji-shan CHANG Yang LIU Rui NI Yong-jing |
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| Institution: | National Center of Engineering and Technological Research for Wheat, Henan Agricultural University, Zhengzhou 450002, China |
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| Abstract: | Near the HMW-glutenin gene of wheat (Triticum aestivum), there is a locus (temporarily named TaXa) encoding LRR-receptor-like protein kinase, which is homologous to disease resistance protein Xa21 of rice (Oryza sativa). Through RT-PCR approach, a cDNA clone of ZS2002 was isolated from the orthologous locus of TaXa in Triticum turgidum. ZS2002 was 3 081 bp long and encoding a peptide composed of 1 026 amino acid. The protein included N-terminal conserved sequence, LRR domains, a transmembrane region and a serine/threonine protein kinase domain. ZS2002 was expressed in root, stem, leaf and spike. The transcribing in seedling leaves was significantly enhanced by Blumeria graminis f.sp. tritici. TaXa gene might play a role in powdery mildew resistance reaction in Triticum. |
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| Keywords: | Triticum turgidum leucine-rich repeat (LRR) receptor-like protein kinase (RLK) cloning characterization |
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