水稻白叶枯病成株抗性与过氧化氢含量及几种酶活性变化的关系

 分析了水稻白叶枯病成株抗性与过氧化氢(H₂O₂)含量及过氧化物酶(POD)、多酚氧化酶(PPO)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性变化的关系。试验结果表明:接种白叶枯病菌T7133后,苗期与成株期植株体内H₂O₂含量上升以及POD、PPO和SOD活性增加。与苗期叶片相比,成株期叶片H₂O₂含量高,且PPO和SOD的酶活性增强,而POD的酶活性则降低。苗期和成株期CAT的酶活性均低于对照,成株期比苗期CAT活性更低。这些结果表明,H₂O₂、PPO、SOD和CAT可能与水稻白叶枯病成株抗性之间存在一定的关联,而POD则没有直接关系。

The change in hydrogen peroxide content and enzyme activity related to adult plant resistance to Rice bacterial blight

The concentration of hydrogen peroxide (H_2O_2) and the activities of peroxidase (POD), polyphenol oxidase (PPO), superoxide dismutase (SOD) and catalase (CAT) were measured in rice leaves inoculated with Xanthomonas oryzae pv. oryzae T7133 or water at seedling and adult plant stage, respectively. Inoculation with T7133 on leaves of both seedlings and adult plants resulted in an increase in the concentration of H_2O_2 and the activities of POD, PPO, and SOD. The concentration of H_2O_2 and the activities of PPO and SOD were higher in adult plants than in seedlings, whereas the POD activity was lower. The activity of CAT in leaves of both seedlings and adult plants inoculated with T7133 was lower than that in water-treated control, and the adult plants showed much lower activity compared to the seedlings. The results suggest the involvement of H_2O_2, PPO, SOD, and CAT, but not of POD, in adult plant resistance against rice bacterial blight.