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| 华重楼斑枯病病原鉴定 | |
| 引用本文： | 伏荣桃,陈诚,王剑,陈雪娟,罗曦,卢代华.华重楼斑枯病病原鉴定[J].植物病理学报,2020,50(3):261-266. |
| 作者姓名： | 伏荣桃 陈诚 王剑 陈雪娟 罗曦 卢代华 |
| 作者单位： | 四川省农业科学院植物保护研究所, 成都 610066; 农业部西南作物有害生物综合治理重点实验室, 成都 610066 |
| 基金项目： | 四川省科技计划项目(2018JY0111);四川省财政创新能力提升工程项目(2016GYSH-014);四川省水稻创新团队项目“病虫害绿色防控岗位” |
| 摘 要： | 2018年,四川汶川县重楼种植区的华重楼出现一种新的病害—华重楼斑枯病,该病害主要危害叶片,发生率为35%,天气适宜时可引起整株叶片枯死。为鉴定引起四川汶川县华重楼斑枯病的病原菌,本试验采用组织分离法对病原菌进行分离,利用科赫氏法则验证其致病性,依据菌株的形态学和基于rDNA-ITS和RPB2基因序列分析对病原菌进行鉴定。结果表明,分离菌株的菌落形态、分生孢子器和分生孢子形态与Didymella sp.相似;经分子生物学鉴定,该菌ITS-RPB2基因序列与亚隔孢壳属D. glomerata(登录号为FJ427013、GU371781)的同源性为100%。因此,将引起重楼斑枯病的病原菌鉴定为D. glomerata。 |
| 关 键 词： | 华重楼 斑枯病 亚隔孢壳属 病原菌鉴定 |
| 收稿时间： | 2019-07-06 |
Identification of the pathogen causing spot blight on Paris polyphylla Smith var.chinensis |
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| FU Rong-tao,CHEN Cheng,WANG Jian,CHEN Xue-juan,LUO Xi,LU Dai-hua.Identification of the pathogen causing spot blight on Paris polyphylla Smith var.chinensis[J].Acta Phytopathologica Sinica,2020,50(3):261-266. | |
| Authors: | FU Rong-tao CHEN Cheng WANG Jian CHEN Xue-juan LUO Xi LU Dai-hua |
| Institution: | Institute of Plant Protection, Sichuan Academy of Agricultural Science, Chengdu 610066, China; Key Laboratory of Integrated Pest Management on Crops in Southwest, Ministry of Agriculture Science, Chengdu 610066, China |
| Abstract: | In 2018, a new spot blight disease on leaf of Paris polyphylla Smith var. chinensis was found with the incidence of about 35% or the whole leaf collapsed at favorite whether conditions in Wenchuan county, Sichuan Province. The causal agent, was isolated by tissue isolation method, and pathogenicity was tested follo-wing reisolation of the pathogen from the diseased leaves to satisfy Koch′s rule. The pathogen was identified based on morphological characteristics and sequence analysis of rDNA-ITS region and RPB2 gene. The results showed that the colony morphology, pycnidia and conidia of the isolates were similar to those of Didymella sp.. The sequences of ITS-RPB2 genes showed 100% identities with D. glomerata (No. FJ427013 and GU371781). Therefore, D. glomerata was identified as the pathogen causing the spot blight on leaf of Paris polyphylla Smith var. chinensis. |
| Keywords: | Paris polyphylla Smith var chinensis spot blight Didymella pathogen identification |
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