| 大豆灰斑病菌生理小种同功酶分析 |
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| 引用本文： | 袁凤杰,杨庆凯.大豆灰斑病菌生理小种同功酶分析[J].植物病理学报,1998,28(1):39-42. |
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| 作者姓名： | 袁凤杰 杨庆凯 |
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| 作者单位： | 东北农业大学大豆研究所, 哈尔滨 150030 |
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| 摘 要： | 本文首次利用聚丙烯酰胺凝胶电泳技术对黑龙江省大豆灰斑病菌10个生理小种进行了酯酶等5种同功酶及可溶性蛋白的分析。分析结果表明各供试小种间存在着同功酶表型异质性。小种间相似系数在0.22~0.96之间,且分析出4号小种与其它小种亲缘关系最远,具有特殊的遗传背景。进一步从生化角度证明灰斑病菌致病力有一定的差异。同时建议制定一套同功酶标准图谱作为生产上监测生理小种毒力变异工作的辅助性手段。
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| 关 键 词： | 大豆灰斑病菌 生理小种 同功酶 |
ISOZYME PATTERNS OF PHYSIOLOGICAL RACES OF CERCOSPORIDUM SOJINUM |
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| Yuan Fengjie,Yang Qingkai.ISOZYME PATTERNS OF PHYSIOLOGICAL RACES OF CERCOSPORIDUM SOJINUM[J].Acta Phytopathologica Sinica,1998,28(1):39-42. |
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| Authors: | Yuan Fengjie Yang Qingkai |
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| Institution: | Soybean Research Institute of Northeast Agricultural University, Harbin 150030 |
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| Abstract: | The soluble mycoprotein and Esterase etc isozyme patterns of 10 physiological races of Cercosporidium sojinum were analyzed and compared with polyacrylamide gel electrophoresis. Significant difference of electropheretic pattern was detected among 10 races. In general, the similar coefficient(sc) among 10 races were about 0.22~0.96,Race 4 was more far related to the other races, An average coefficient of similarity was low. The results indicated that the isozyme pattern of physiological races of Cercosporidium sojinum were useful genetic markers which were independent of virulence. |
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| Keywords: | Cercosporidium sojinum Physiological race Isozyme pattern |
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