几种蚜虫对MDMV传毒效率及其口针中病毒附着位点(VAS)的免疫荧光标记

 本研究在测定了5种蚜虫对玉米矮花叶病毒(MDMV)传播效率的基础上,采用免疫荧光(FITC)标记方法,观察到蚜虫口针中MDMV附着位点(VAS)的存在。结果发现不同蚜虫之间的传毒效率及VAS有一定的差异。其中,以麦二叉蚜(Schizaphis graminum)的传毒效率最高,达66.8%。并且其VAS也最明显,位于口针末端约50μm处。通过试验发现,尽管在VAS存在的情况下,用提纯的不含HC的MDMV进行蚜虫传毒试验,其传毒率为零。只有当HC与MDMV共同存在的情况下才可有效传播。至于蚜虫是如何释放病毒的还不清楚。根据试验结果初步认为,蚜虫对MDMV的传播是一个"识别-吸附-释放"的过程。

TRANSMISSION RATE AND LOCATION OF MAIZE DWARF MOSAIC VIRUS ATTACHMENT SITES (VAS) IN THE STYLETS OF APHIDS BY IMMUNOFLUORESENCE LABEL

Transmission rate and location of maize dwarf mosaic virus attachment sites (VAS) in the stylets of aphids by immunofluoresence label were observed. The results showed that the rate of transmission of MDMV is different between five aphids, including schizaphis graminum, Rhopalosiphum padi, Rhopalosiphum maidis, Macrosiphum avenae, and Myzus persicae Among them Schizaphis graminum has the highest transmission rate. MDMV VAS is different from different aphids stylet. VAS is quite obvious in S.graminum stylet, locating on the maxillae at about 50 μm from the tip. In addition, experiment shows that the HC-Pro transmiasion of MDMV is in accordance to the procedure of "recognition-adhesion-release".