FpStuA参与假禾谷镰孢的产孢和致病性

 假禾谷镰孢是一种土传真菌,其引起的小麦茎基腐病已成为威胁我国小麦生产的重要病害。APSES蛋白是真菌中保守存在的一类转录因子,参与多种细胞生理过程。本研究,我们在假禾谷镰孢中鉴定到StuA同源蛋白FpStuA。qRT-PCR分析发现FpStuA在假禾谷镰孢分生孢子和侵染阶段诱导表达。通过PEG介导的原生质体转化方法获得3个FpStuA基因缺失的突变体。与假禾谷镰孢野生型菌株相比,Δfpstua突变体的生长速度明显减慢,气生菌丝减少;分生孢子的产生比野生型减少70%,且Δfpstua突变体分生孢子变短、分隔减少;Δfpstua突变体对大麦叶片、小麦胚芽鞘和小麦根的致病性均显著降低,脱氧雪腐镰刀菌烯醇(DON)合成明显减少。综上结果,转录因子FpStuA对假禾谷镰孢的生长、产孢和致病性都非常重要。

FpStuA is involved in conidiation and pathogenicity of Fusarium pseudograminearum

Fusarium pseudograminearum is a soil-borne fungal pathogen causing Fusarium crown rot, which has been becoming one of major pathogens that threaten wheat production in China. The APSES family is found exclusively in fungi and has been reported to control several cellular processes in these organisms. In this study, we identified a conserved StuA ortholog FpStuA, which was highly expressed in conidia and infection stages. Three FpStuA deletion mutants were obtained by polyethylene glycol (PEG)-mediated protoplast stable transformation. Compared with the wild-type strain, Δfpstua mutants grew relatively slowly and had few aerial hyphae.Δfpstua mutants displayed about 70% reduction in conidiation compared to the WT. Moreover, conidia of Δfpstua mutants were smaller and fewer separation. Pathogenicity assays showed that Δfpstua mutants were dramatically decreased in virulence on barley leaves, wheat coleoptiles and wheat roots. Furthermore, deoxynivalenol production was significantly reduced in Δfpstua mutants. Taken together, these results indicated that FpStuA was important for growth, conidiation and virulence in F. pseudograminearum.