| 菊欧文氏菌分子检测技术的研究 |
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| 引用本文： | 刘鹏,黄国明,刘勇,崔铁军.菊欧文氏菌分子检测技术的研究[J].植物病理学报,2007,37(6):584-587. |
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| 作者姓名： | 刘鹏 黄国明 刘勇 崔铁军 |
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| 作者单位： | 天津出入境检验检疫局, 天津 300456 |
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| 摘 要： | 蝴蝶兰细菌性软腐病对蝴蝶兰的生长危害严重, Erwinia chrysanthemi(菊欧文氏菌)、Erwinia carotovora subsp. carotovora(胡萝卜软腐欧文氏菌胡萝卜软腐亚种)是引起蝴蝶兰软腐病的主要病原细菌, 其中E.chrysanthemi被列入我国三类检疫性有害生物。本文对菊欧文氏菌分子检测技术进行了研究, 设计出针对该病原细菌的特异性引物, 应用实时荧光PCR方法检测样品中存在的菊欧文氏菌, 检测灵敏度达到102cfu/mL。
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| 关 键 词： | 菊欧文氏菌 特异性引物 检测 |
| 文章编号： | 0412-0914（2007）06-0584-04 |
| 收稿时间： | 2006-08-28 |
| 修稿时间： | 2007-04-16 |
Molecular detection of Erwinia chrysanthemi |
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| LIU Peng,HUANG Guo-ming,LIU Yong,CUI Tie-jun.Molecular detection of Erwinia chrysanthemi[J].Acta Phytopathologica Sinica,2007,37(6):584-587. |
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| Authors: | LIU Peng HUANG Guo-ming LIU Yong CUI Tie-jun |
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| Institution: | Tianjin Entry-Exit Inspection and Quarantine Bureau, Tianjin 300456, China |
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| Abstract: | Bacterial soft rot causes great loss to the growth of butterfly orchid.Erwinia chrysanthemi and Erwinia carotovora subsp.carotovora are the main bacterial pathogen led to rot disease.In the study,the mole-cular technology had been primarily established to detect E.chrysanthemi,the potential serious quarantine pathogen.The specific primers was designed to the detection of E.chrysanthemi and real-time fluorescent PCR method could achieve high sensitivity. |
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| Keywords: | Erwinia chrysanthemi specific primers detection |
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