| 红掌细菌性疫病病原菌的PCR特异性检测 |
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| 引用本文： | 卢梦玲,周晓云,曾伟达,陈一新,刘琼光.红掌细菌性疫病病原菌的PCR特异性检测[J].植物病理学报,2012,42(1):1-9. |
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| 作者姓名： | 卢梦玲 周晓云 曾伟达 陈一新 刘琼光 |
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| 作者单位： | 1 华南农业大学资源环境学院, 广州510642; 2 广州花卉研究中心, 广州510360 |
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| 基金项目： | 广东省科技计划项目(2010B020401002);广州市科技计划项目(4200-F10028) |
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| 摘 要： | 红掌细菌性疫病(Xanthomonas axonopodis pv. dieffenbachiae,简称Xad)是红掌等天南星科花卉毁灭性病害,该病通过带菌种苗调运不断在我国扩散蔓延,国内尚未有检测方法。通过筛选和重新设计引物,建立了Xad 的PCR 检测方法,结果表明,利用引物Xad-F / Xad-R 进行PCR,能扩增出检测Xad 的特异性DNA 片断,其灵敏度可达1 × 102 CFU / mL,DNA 的最低检出限为0. 44 ng / μL,可用于红掌苗的带菌检测和红掌细菌病害的鉴定。
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| 关 键 词： | 红掌 Xanthomonas axonopodis pv. dieffenbachiae PCR 检测 |
Specific PCR detection of Xanthomonas axonopodis pv. dieffenbachiae |
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| LU Meng-ling
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ZHOU Xiao-yun
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ZENG Wei-da
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CHEN Yi-xin
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LIU Qiong-guang.Specific PCR detection of Xanthomonas axonopodis pv. dieffenbachiae[J].Acta Phytopathologica Sinica,2012,42(1):1-9. |
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| Authors: | LU Meng-ling ZHOU Xiao-yun ZENG Wei-da CHEN Yi-xin LIU Qiong-guang |
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| Institution: | 1 College of Natural Resource & Environment South China Agricultural University, Guangzhou 510642, China; 2 Guangzhou Flowering Plant Research
Centre,Guangzhou 510360, China |
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| Abstract: | Bacterial blight of Anthurium caused by Xanthomonas axonopodis pv.dieffenbachiae(Xad) is a destructive disease.The disease is spreaded with plant seedlings and no method for the pathogenic bacteria detection in our country.In this study,a PCR system for Xad detection with the specific primers Xad-F/XadR was developed.The results showed that Xad could be detected specifically by the system and the thresholds of PCR were 1×102 CFU/mL for Xad suspension and 0.44 ng/μL for its DNA.The method developed in this study is effective to detect and identify the pathogen in Anthurium plants. |
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| Keywords: | Anthurium andraeanum Xanthomonas axonopodis pv dieffenbachiae PCR detection |
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