转隐地蛋白基因烟草的抗病性及遗传分析

 对转隐地蛋白(Cryptogein)基因烟草植株进行了抗病性测定、抗病分子机理及遗传分析的研究。结果表明:80%以上的转基因植株对烟草上3种重要病原菌的抗病性均增强,且能稳定遗传;转基因烟草植株的抗病性与整合的拷贝数成负相关,即绝大多数高度抗病的植株只含有1个拷贝,而感病植株一般为2~3个拷贝;部分转基因烟草植株Northern杂交分析表明:病程相关蛋白和渗透调节蛋白等防卫反应相关基因的表达丰度与转基因植株的抗病性存在着一定的正相关性,表达丰度越高,抗病性越强;综合农艺性状考察表明Cryptogein基因对烟草植株的生长有显著的促进作用。

Disease resistance and inheritance analysis of Cryptogein transgenic tobacco plants

The transgenic tobacco plants were challenged separately, with black shank fungal Phytophthora parasitica var. nicotianae , brown spot fungal Alternaria alternata, and wild fire bacterial Pseudomonas syringae pv. tabaci . Most (80%) transgenic tobacco plants exhibited high level resistance to these pathogens, although with variations of individuals. Correlation analysis between the integrated copy numbers and disease resistance to black shank showed that highly resistant plants usually contained only one copy, and the susceptible plants contained 2-3 copies. Northern blot analysis showed that the expression level of PR1 gene and Osmotin gene varied among transgenics and had a positive correlation with the disease resistance. Observation of agronomic traits of some transgenic tobacco plants indicated that the Cryptogein gene could promote growing of transgenic tobacco plants.