抗大麦黄矮病毒GPV株系小麦异附加系Z1抗性基因的RAPD标记

 经生物学方法鉴定,小麦-中间偃麦草异附加系Z1高抗我国特有的大麦黄矮病毒GPV株系,用104个随机引物对Z1及其亲本中7902的基因组进行RAPD分析,发现其中的引物OPS-16能从Z1中扩增1个约1.7 K b的特异性片段。用引物OPS-16扩增Z1、中7902、中间偃麦草、中5,同样也从Z1、中间偃麦草、中5中能稳定地扩增此1.7 Kb片段。对Z1的抗、感病单株的自交后代进行RAPD分析,发现在所测的抗病株的自交后代中能稳定地扩增此1.7 Kb片段,而在感病株的自交后代中则不能扩增。

RAPD MARKER OF RESISTANCE GENE TO STRAIN GPV OF BYDV IN WHEAT ADDITION LINE Z1

The wheat-Elytrigia intermedia addition line Z1 was identified to be highly resistant to strain GPV of BYDV, and this strain exists only in China. The genomes of resistant lines and susceptible lines were analyzed by RAPD using 104 random primers. It was found that primer OPS16 can amplify a specific 1.7 Kb fragment from genome of Z1.When Z1, Zhong7902, Elytrigia intermedia and Zhong5 were amplified using primer OPS16, this specific 1.7 Kb fragment was also produced in Elytrigia intermedia, Zhong5 and Z1. When selfbred progenies of resistant and susceptible Z1 individuals were amplified using primer OPS16, this specific 1.7 Kb fragment was amplified in progenies of resistant individuals of Z1, but not in progenies of susceptible individuals.