| 应用组织培养技术离体筛选枸杞抗根腐病变异体的研究 |
| |
| 引用本文： | 曹有龙,贾勇炯,赵军.应用组织培养技术离体筛选枸杞抗根腐病变异体的研究[J].植物病理学报,1999,29(2):163-168. |
| |
| 作者姓名： | 曹有龙 贾勇炯 赵军 |
| |
| 作者单位： | 四川大学生命科学学院, 成都 610064 |
| |
| 摘 要： | 用宁夏枸杞-宁杞1号的髓组织进行离体培养,诱导产生胚性愈伤组织,并用60Co-γ射线进行诱变,以枸杞根腐菌尖孢镰刀菌(Fusarium oxysporum Schlecht)菌株产生的培养滤液为选择剂,筛选抗性变异体,在含毒素60%的条件下,一次性获得抗根腐病毒素的愈伤组织,抗性愈伤组织经稳定性分析,其抗性稳定。抗性愈伤组织在含30%的毒素培养基中可分化成再生植株,共获得25株,其叶片经用根腐菌分生孢子液接种鉴定,有6株抗病;再生植株经过脯氨酸、叶绿素含量分析,二者含量都有所提高;经过过氧化物同工酶酶谱分析,抗性再生植株酶谱带数有增加;说明经筛选得到的抗性再生植株是抗根腐病的变异体。
|
| 关 键 词： | 枸杞 离体培养 诱变 抗病变异体 再生植株 |
| 修稿时间： | 1997-03-17 |
IN VITRO SCREENING OF RESISTANT VARIANTS OF Lycium barbarum L. TO ROOT ROT DISEASE |
| |
| Cao Youlong,Jia Yongjiong,Zhao Jun,Chen Xiaobin,Chen Fang.IN VITRO SCREENING OF RESISTANT VARIANTS OF Lycium barbarum L. TO ROOT ROT DISEASE[J].Acta Phytopathologica Sinica,1999,29(2):163-168. |
| |
| Authors: | Cao Youlong Jia Yongjiong Zhao Jun Chen Xiaobin Chen Fang |
| |
| Institution: | College of Life Science, Sichuan University, Chengdu 610064 |
| |
| Abstract: | To obtain resistant variants, embryonic calli from pith culture were irradiated with 60Co-γ ray. Resistant calli were screened of cultural filtrate containing 60% toxin of Fusarium oxysporum. Plantlet were then regenerated on differentiation medium containing 30% of toxin. Physiological and biochemical assay showed that both proline and chlorophyll contents increased in resistant variants. Meanwhile, the bands of peroxidase isozyme on PAGE increased as well. Inoculation with suspension of conidium demonstrated that 6 regenerated plantlets were resistant to root rot disease. |
| |
| Keywords: | Lycium barbarum in vitro culture mutagenesis resistant variants regenerated plantlets |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《植物病理学报》浏览原始摘要信息 |
| 点击此处可从《植物病理学报》下载免费的PDF全文 |
|
