拟南芥BOI 基因在抗氧化胁迫和细胞死亡中的功能分析

 本文利用BOI(Botrytis Susceptible 1 Interactor)基因敲减(knock-down) 株系、过量表达株系以及烟草瞬时表达系统,研究了BOI 基因在细胞程序化死亡(PCD)中的作用以及BOI 蛋白中RING 和WRD 结构域的功能。结果表明,BOI 基因的表达下调导致抗氧化胁迫能力下降,弱化了对活性氧介导的PCD 抑制作用;BOI 过量表达或瞬间表达可以增强对活性氧介导和α-吡啶甲酸诱导的PCD 的抑制作用。进一步研究发现,BOI 蛋白中的RING 结构域是BOI 抑制PCD 所必需的,WRD 结构域与BOI 对PCD 的抑制作用关系不大。

Functional analysis of Arabidopsis Botrytis Susceptible 1 Interactor in oxidative stress and programmed cell death

Arabidopsis BOI(Botrytis Susceptible 1 Interactor) encodes a RING protein with ubiquitin E3 ligase activity and plays important roles in defense against Botrytis cinerea and response to abiotic stress.In this study,the function of BOI in programmed cell death(PCD) was analyzed using knock-down and overexpression lines and the functions of the conserved RING and WRD domains of BOI protein in PCD was ex-amined using constructs containing different domains of BOI protein.Down regulation of BOI led to reduced tolerance to oxidative stress and attenuated inhibitory effect on PCD induced by reactive oxygen species(ROS).Overexpression of BOI in stable transgenic lines and transient expression of BOI in tobacco leaves resulted in increased inhibitory effect on ROS-and α-picolinic acid(an inducer of PCD)-induced PCD.Further studies indicated that the RING domain but not the WRD domain in BOI protein was probably required for PCD inhibition.