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Diversity of Phytophthora infestans from Poland
Authors:M Chmielarz  S Sobkowiak  K D?bski  D E L Cooke  M B Brurberg  J ?liwka
Institution:1. M?ochów Research Centre, Plant Breeding and Acclimatization Institute – National Research Institute, , 05‐831 M?ochów;2. Warsaw University of Life Sciences, , 02‐787 Warszawa, Poland;3. The James Hutton Institute, , Invergowrie, Dundee, DD2 5DA UK;4. Bioforsk – Norwegian Institute for Agricultural and Environmental Research, , 1432 ?s, Norway
Abstract:A collection of 96 Polish isolates of Phytophthora infestans sampled in the years 2006, 2008 and 2009 were analysed using phenotypic and genotypic markers. Mating type, virulence, resistance to metalaxyl, mitochondrial haplotype and polymorphism at 12 simple sequence repeat (SSR) loci were determined. The majority of isolates were of the A1 mating type, mitochondrial haplotype Ia and sensitive to metalaxyl. Virulence factors against potato R genes R1, R3, R4, R7, R10 and R11 were present in most isolates. Genotyping using SSR markers revealed high genetic diversity within the Polish P. infestans population. Amongst the 96 isolates 66 unique genotypes were identified, 49 of which were observed only in single isolates. Eight isolates of the genotype 13_A2 lineage that has been reported in other parts of Europe were also found in Poland. The implications of these results are discussed.
Keywords:genotyping  late blight  microsatellites  population  potato  simple sequence repeats
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