Selenium and vitamin E, natural antioxidants, protect rat cerebral cortex against dimethoate-induced neurotoxicity |
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Authors: | Ibtissem Ben AmaraNejla Soudani Ahmed Hakim Afef TroudiKhaled Mounir Zeghal Tahia BoudawaraNajiba Zeghal |
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Institution: | a Animal Physiology Laboratory, Sfax Faculty of Science, University of Sfax, BP 1171, 3000 Sfax, Tunisia b Laboratory of Pharmacology, Medicine Faculty, University of Sfax, 3029 Sfax, Tunisia c Anatomopathology Laboratory, CHU Habib Bourguiba, University of Sfax, 3029 Sfax, Tunisia |
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Abstract: | Pesticides have been used in agriculture to enhance food production by eradicating unwanted insects and controlling disease vectors, nevertheless occupational exposure to high levels of these compounds can lead to neurodegenerative disorders, characterized by serious oxidative and neurotoxic effects. However, there is a lack of consensus as to which determinations are best used to quantify future risks arising from xenobiotic exposure and natural antioxidant interventions. Our study aims to determine the potential ability of selenium and/or vitamin E, used as nutritional supplements, to alleviate oxidative stress in cerebral cortex tissue induced by dimethoate, an organophosphorus pesticide. Adult Wistar rats were exposed either to dimethoate (0.2 g/L of drinking water), dimethoate + selenium (0.5 mg/kg of diet), dimethoate + vitamin E (100 mg/kg of diet), or dimethoate + selenium + vitamin E, for 30 days. Exposure to dimethoate increased malondialdehyde levels, protein carbonyl groups and advanced oxidation protein products, while Na+K+-ATPase, acetylcholinesterase and butyrylcholinesterase activities decreased in the cerebral cortex. An increase in glutathione peroxidase, superoxide dismutase and catalase activities and a decrease in glutathione, non-protein thiols and vitamin C levels were observed. Administration of selenium and/or vitamin E through the diet in dimethoate treated rats ameliorated the biochemical parameters cited above. The histological findings confirmed the biochemical results. The model of this study that we employed characterized the relationships between dimethoate-induced neurotoxicity and its alleviation by natural antioxidants like selenium and vitamin E. These elements may be considered beneficial for the protection of cerebral cortex against injury induced by dimethoate. |
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Keywords: | AChE acetylcholinesterase AOPP advanced oxidation of protein products ATPase adenosine triphosphatase BuChE butyrylcholinesterase CAT catalase DM dimethoate GPx glutathione peroxidase GSH glutathione MDA malondialdehyde OP organophosphorus SOD superoxide dismutase NPSH non-protein thiol LPO lipid peroxidation ROS reactive oxygen species PCO protein carbonyls Se selenium Vit E vitamin E |
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