Modulatory effect of vitamins A, C and E mixtures against tefluthrin pesticide genotoxicity in rats

The present experiments to be reported were declared the effect of mixtures from the three antioxidant vitamins (A, C and E) on some hematologic parameters, liver and testes nucleic acid system, blood immune-system, chromosomal aberration and sperm-shape of tefluthrin (LD₅₀) intoxicated rats. A total of 90 male albino rats were used divided into six groups (15 rats each).Oral ingestion of technical tefluthrin significantly decreased the count of RBCs, Hb content and stimulated plasma LDH activity, also reduced the levels of blood immuno globulins (IgG, IgA and IgM). Under the effects of tefluthrin liver and tests nucleic acids (RNA and DNA) contents were decreased and the activities of nucleases (RNAase and DNAase) were also inhibited relative to these values of normal healthy control group.Chromosomal aberrations and sperm-shape abnormalities were significantly increased after ingestion of tefluthrin, compared to the control group. Mitotic index (MI) and sperm count was decreased significantly due to the potential cytotoxicity of tefluthrin.Treatment of intoxicated rats with antioxidant vitamins (A, C and E) mixtures reduced the harmful influences of tefluthrin. Blood IgG, IgA and IgM levels, RBCs count and Hb content were improved relative to normal healthy animal group.The frequency of chromosome aberrations in bone marrow cells of intoxicated rats treated with vitamins as well as the frequency of sperm abnormalities were decreased and readjusted near to that of the healthy control animals, also MI and sperm counts were increased significantly near to the control group after vitamins ingestion as treatments.It is interesting to note that, the ingestion of vitamins mixtures readjusted and normalized the hematologic parameters around those of normal healthy control. In case of liver and nucleic acids system, the disturbed effects of the pesticide induction of RNA and DNA contents as well as RNAase and DNAase activities were alleviated by the treatments with the present antioxidative vitamins mixtures, in which the nucleic acids contents and nucleases activities of liver and testes of tefluthrin intoxicated rats were ameliorated and normalized compared with those of normal control.