Improved method to induce sporulation of <Emphasis Type="Italic">Colletotrichum gloeosporioides</Emphasis>, causal fungus of grape ripe rot |
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Authors: | Koichi Suzaki |
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Institution: | (1) Grape and Persimmon Research Station, National Institute of Fruit Tree Science, National Agriculture and Food Research Organization, Akitsu 301-2, Higashihiroshima Hiroshima, 739-2494, Japan |
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Abstract: | Colletotrichum
gloeosporioides and C. acutatum are causal agents of grape ripe rot, but with available methods, sporulation of C.
gloeosporioides on plate media has been unstable and inferior to that of C. acutatum. To facilitate studies on C. gloeosporioides, I developed an improved method to induce conidiation of this fungus. Isolates of C. gloeosporioides were pre-cultured in potato dextrose broth for 1 week, then pulverized in whole broth. The homogenate was then spread on
diluted oatmeal agar (15–20% commercial oatmeal agar medium, 1.5% agar) plates. After the plates were cultured at 25°C under
continuous light for another week, the C. gloeosporioides isolates sporulated stably on the plate medium. |
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