| 苏云金芽胞杆菌分离过程中无晶体芽胞杆菌的分析 |
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| 引用本文： | 周建桥,束长龙,邵高祥,李海涛,宋福平,张杰.苏云金芽胞杆菌分离过程中无晶体芽胞杆菌的分析[J].中国生物防治,2011,27(1):6-10. |
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| 作者姓名： | 周建桥 束长龙 邵高祥 李海涛 宋福平 张杰 |
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| 作者单位： | 1. 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京,100193
2. 东北农业大学生命科学院,哈尔滨,150030 |
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| 基金项目： | 国家“863”计划(2006AA10A212); 国家转基因生物产业化专项(2009ZX08009-030B,2009ZX08009-031B) |
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| 摘 要： | 本研究选择了在苏云金芽胞杆菌Bacillus thuringiensis(Bt)分离过程中发现的与Bt芽胞相似、没有伴胞晶体的8株芽胞杆菌,对其种属、质粒等生物学特性和杀虫活性进行了初步研究.对分离菌株所产芽胞进行形态观察及质粒图谱分析,发现其与Bt相似;但肽指纹图谱分析和16S rDNA聚类研究表明所分离菌株与蜡样芽胞杆菌B.cereus(Bc)匹配最高;杀虫活性测定表明这8株菌对猿叶甲Colaphellus bowringi幼虫均无毒杀作用,而15-3、W23-1和W25-3 3个菌株及其培养上清液对小菜蛾Plutella xylostella幼虫有一定的杀虫活性,说明它们能产生活性物质.这些结果说明在Bt菌株的分离过程中要对无晶体菌株加以重视,其含有的活性物质具有潜在的研究和利用价值.
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| 关 键 词： | 苏云金芽胞杆菌 蜡样芽胞杆菌 肽指纹图谱 新型杀虫活性物质 |
Analysis of Bacillus without Protein Crystals during Isolation of Bacillus thuringiensis |
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| ZHOU Jian-qiao
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SHU Chang-long
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SHAO Gao-xiang
,
LI Hai-tao
,
SONG Fu-ping
,
ZHANG Jie.Analysis of Bacillus without Protein Crystals during Isolation of Bacillus thuringiensis[J].Chinese Journal of Biological Control,2011,27(1):6-10. |
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| Authors: | ZHOU Jian-qiao SHU Chang-long SHAO Gao-xiang LI Hai-tao SONG Fu-ping ZHANG Jie |
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| Institution: | ZHOU Jian-qiao1,SHU Chang-long1,SHAO Gao-xiang1,LI Hai-tao2,SONG Fu-ping1,ZHANG Jie1(1.State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of AgriculturalSciences,Beijing 100193,2.College of Life Sciences,Northeast Agricultural University,Harbin 150030,China) |
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| Abstract: | Three aspects including species,biological characteristic and insecticidal activity of eight strains of Bacillus spp.with spores similar to B.thuringiensis and without protein crystals were analyzed.Both spores morphology and plasmid pattern of these Bacillus spp.showed similarity with those of B.thuringiensis,however,assay of Peptide Mass Fingerprinting and species identification by 16S rDNA indicated they shared the highest homology with B.cereus. Bioassay results of the eight strains against larvae of Co... |
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| Keywords: | Bacillus thuringiensis Bacillus cereus Peptide Mass Fingerprinting novel insecticidal substance |
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