| 枯草芽孢杆菌菌株Tpb55的安全性毒理学研究 |
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| 引用本文： | 张玉芹,高加明,张成省,孔凡玉,王静,冯超.枯草芽孢杆菌菌株Tpb55的安全性毒理学研究[J].中国生物防治,2013(4):601-606. |
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| 作者姓名： | 张玉芹 高加明 张成省 孔凡玉 王静 冯超 |
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| 作者单位： | [1]中国农业科学院烟草研究所／烟草行业病虫害监测与综合治理重点实验室,青岛266101 [2]中国烟草总公司山东省公司,济南250000 [3]中国烟草总公司湖北省公司,武汉430000 |
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| 基金项目： | 基金项目：中国烟草总公司项目（2012YN42）;青岛市科技支撑计划（09-1-1-90-nsh）;湖北省烟草公司重点科技项目（027Y2012.083） |
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| 摘 要： | 对枯草芽孢杆菌菌株Tpb55的安全性进行了合理的评价，就菌粉Tpb55对试验动物进行了相关的毒理学试验。结果表明，菌株Tpb55对小鼠、大鼠急性经口LD50大于5000mg·kg-1，对大鼠急性经皮LD50大于5000mg·kg-1；对兔皮肤刺激强度为无刺激性，眼刺激平均指数48h后为0，眼刺激强度为无刺激性；对豚鼠致敏率为0，属I级弱致敏物，无致敏性；对小鼠骨髓嗜多染红细孢微核以及性染色体及常染色体单价体等3项致突变试验为阴性，说明菌株Tpb55没有致突变作用。
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| 关 键 词： | 枯草芽孢杆菌 安全性评估 毒理学试验 |
Toxicological and Safety Evaluation of Bacillus subtilis Strain Tpb55 |
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| ZHANG Yuqin,＇,GAO Jiaming,ZHANG Chengsheng,KONG Fanyu,WANG Jing,FENG Chao.Toxicological and Safety Evaluation of Bacillus subtilis Strain Tpb55[J].Chinese Journal of Biological Control,2013(4):601-606. |
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| Authors: | ZHANG Yuqin ＇ GAO Jiaming ZHANG Chengsheng KONG Fanyu WANG Jing FENG Chao |
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| Institution: | 1 (1. Tobacco Research Institute, Chinese Academy of Agricultural Sciences/Key Laboratory of Tobacco Pest Monitoring and Integrated Management, State Tobacco Monopoly Bureau, Qingdao 266101; 2. Shandong Corporation of China National Tobacco Corporation, Ji＇nan 250000; 3. Hubei Corporation of China National Tobacco Corporation, Wuhan 430000, China) |
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| Abstract: | The toxicological safety of Bacillus subtilis strain Tpb55 was evaluated. The results showed that the acute oral and cute dermal toxicity of LDs0 of Tpb55 was greater than 5000 mg-kg-1, respectively. No irritation to rabbit skin and eye was found, and the rate of dermal sensitization was 0 after 48 h. Thus, it can be concluded that the acute toxicity of Tpb55 both through mouth and skin was in lower level with no irritations to rabbit skin and eye detected. Its dermal hypersusceptibility rate was zero for the guinea pigs, being a weak sensitized tar of grade I, there were no significant differences between the experimental groups and the control group in micronucleus assay and sperm shape abnormality. Therefore, the strain Tpb55 is a safe biological bacterium with low toxicity, no irritation, no sensitization and no mutagenic effect. |
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| Keywords: | Bacillus subtilis safety assessment toxicological |
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