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| 短短芽孢杆菌JK-2菌株对番茄枯萎病的抑菌作用及其小区防效 | |
| 引用本文： | 郝晓娟,刘波,谢关林,葛慈斌,林抗美.短短芽孢杆菌JK-2菌株对番茄枯萎病的抑菌作用及其小区防效[J].中国生物防治,2007,23(3):233-236. |
| 作者姓名： | 郝晓娟 刘波 谢关林 葛慈斌 林抗美 |
| 作者单位： | 1. 浙江大学生物技术研究所/水稻生物学国家重点实验室,杭州,310029;福建省农科院生物技术研究所,福州,350003 2. 福建省农科院生物技术研究所,福州,350003 3. 浙江大学生物技术研究所/水稻生物学国家重点实验室,杭州,310029 |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | 对分离自土壤的短短芽孢杆菌JK-2菌株对番茄枯萎病菌的防治效果和抑制作用进行了研究。结果表明：JK-2对番茄枯萎病菌的盆栽防效和田间防效分别为83．82％、74．70％。该菌株能抑制枯萎病菌菌丝的生长，当其无菌滤液终浓度为15％时，对菌丝生长的抑制率达到81．69％。JK-2菌株对病菌孢子萌发也有较强的抑制作用。扫描电镜观察结果：JK-2菌株可造成菌丝消解、产生泡状物、破坏生长点、引起细胞内含物外溢。 |
| 关 键 词： | 拮抗细菌 番茄枯萎菌 抑制作用 生物防治 |
| 文章编号： | 1005-9261（2007）03-0233-04 |
| 修稿时间： | 2006-10-25 |
Inhibition of Brevibacillus brevis JK-2 Strain against the Pathogen of Tomato Fusarium Wilt |
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| HAO Xiao-juan,Liu Bo,XIE Guan-lin,GE Ci-bin,LIN Kang-mei.Inhibition of Brevibacillus brevis JK-2 Strain against the Pathogen of Tomato Fusarium Wilt[J].Chinese Journal of Biological Control,2007,23(3):233-236. | |
| Authors: | HAO Xiao-juan Liu Bo XIE Guan-lin GE Ci-bin LIN Kang-mei |
| Institution: | College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029 |
| Abstract: | Brevibacillus brevis JK-2 isolated from the soil was studied for its biocontrol effect and inhibition against the pathogen of tomato Fusarium wilt. The results showed JK-2 strain exhibited 83.82 % and 74.70% of control effectiveness in pot and field experiments, respectively. JK-2 strongly inhibited mycelium growth of F. oxysporum f. sp. radicis lycopersici. When concentration of the crude culture filtrate was 15%, the inhibition of JK-2 reached 81.69%. It had strong antagonistic effect on spore germi- nation. The results of observation under scanning electron microscope illustrated that JK-2 could dissolve the mycelial, produce bubbles in the cell plasma, destroy the growth points and cause the leak of cytoplasmic components. |
| Keywords: | antagonistic bacterium Fusarium oxysporum f sp radicis lycopersici inhibition biocontrol |
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