| 酶联免疫吸附测定(ELISA)检测苏云金杆菌伴孢晶体蛋白质研究 |
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| 引用本文： | 罗绍彬,阎建平,戴顺英,刘娥英,张用梅,胡名瑞.酶联免疫吸附测定(ELISA)检测苏云金杆菌伴孢晶体蛋白质研究[J].中国生物防治,1987(4). |
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| 作者姓名： | 罗绍彬 阎建平 戴顺英 刘娥英 张用梅 胡名瑞 |
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| 作者单位： | 中国科学院武汉病毒研究所
(罗绍彬，阎建平，戴顺英，刘娥英，张用梅)，中国科学院武汉病毒研究所(胡名瑞) |
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| 基金项目： | 中国农科院生防室主持的75—03—03—01项目课题 |
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| 摘 要： | 用酶联免疫吸附测定(ELISA)检测Bt伴孢晶体毒素蛋白质,最低可检值7.79ng/0.15ml,灵敏、精确,具高度专化性。测定标准品HI—1—S—1980和Bt乳剂产品中伴孢晶体蛋白质含量,分别为108.06mg/g粉剂和4.57—6.17mg/ml乳剂。用标准品与待测样品(Bt乳剂)的晶体蛋白质含量,就可计算出待测样品的相对毒力效价。ELISA与昆虫生测之间有良好的相关性。
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| 关 键 词： | 酶联免疫吸附测定 苏云金杆菌HD—1 Bt乳剂 生物测定 |
ENZYME-LINKED IMMUNOSORBENT ASSAY FOR THE DETECTION AND QUANTIFICATION OF CRYSTAL TOXINS OF BACILLUS THURINGIENSIS |
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| LUO Shaobin YAN Janping DAI Shunying LIU Erying ZHANG Yongmei HU Mingrui.ENZYME-LINKED IMMUNOSORBENT ASSAY FOR THE DETECTION AND QUANTIFICATION OF CRYSTAL TOXINS OF BACILLUS THURINGIENSIS[J].Chinese Journal of Biological Control,1987(4). |
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| Authors: | LUO Shaobin YAN Janping DAI Shunying LIU Erying ZHANG Yongmei HU Mingrui |
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| Abstract: | Enzyme-Linked immunosorbent assay was used to detect and quantitate the insecticidal crystal toxin proteins of B. thuringiensis HD-1 and commercial preparations. The minimal detectable value by indirect method of ELISA was 7.79 ng/0.15ml. The total crystal protein in standard sample HD-1-S-1980 and a commercial Bt emulsion (produced by Hubei Academy of Agricultural Sciences) was measured to be 108.06 mg per gram of dry powder and 4.57-6.17 mg per ml of emulsion, respectively. With these two values, the relative toxicity potency of a commercial emulsion can then be calculated. |
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| Keywords: | ELISA Bacillus thuringiensis HD-1 immunosorbent bioassay |
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