|
|||||
|
|
| 枯草芽孢杆菌C-D6抗菌蛋白对稻瘟病菌的抑菌作用 | |
| 引用本文： | 贾书娟,曾大兴,吴小丽,涂国全.枯草芽孢杆菌C-D6抗菌蛋白对稻瘟病菌的抑菌作用[J].中国生物防治,2011,27(3):362-367. |
| 作者姓名： | 贾书娟 曾大兴 吴小丽 涂国全 |
| 作者单位： | 1. 江西农业大学生物科学与工程学院,南昌330045;深圳职业技术学院化生学院,深圳518055 2. 深圳职业技术学院化生学院,深圳,518055 3. 江西农业大学生物科学与工程学院,南昌,330045 |
| 基金项目： | 深圳市基础研究计划项目资助(2109K3070013) |
| 摘 要： | 枯草芽孢杆菌Bacillus subtilis菌株C-D6及其发酵滤液在平板上对稻瘟病菌Magnaporthe oryzae显示明显的抑制作用。通过20%饱和度硫酸铵沉淀和Sephadex G-75凝胶柱层析分离,获得1个影响病原菌附着胞形成的抗菌蛋白,SDS-聚丙烯酰胺凝胶电泳检测发现该蛋白的分子量为32kD。显微观察发现：用该蛋白处理的孢子虽能完全萌发,但形成串珠状膨大的芽管和色浅、膨大的附着胞,并抑制芽管的正常生长。该蛋白的活性表现具有较好的热稳定性（80℃,30min）和较广的pH适应范围（5.6～9.3）。 |
| 关 键 词： | 枯草芽孢杆菌 抗菌蛋白 稻瘟病菌 附着胞 |
Inhibitory of an Antifungal Protein Produced by Bacillus subtilis C-D6 against Magnaporthe oryzae |
|
| JIA Shu-juan,ZENG Da-xing,WU Xiao-li,TU Guo-quan.Inhibitory of an Antifungal Protein Produced by Bacillus subtilis C-D6 against Magnaporthe oryzae[J].Chinese Journal of Biological Control,2011,27(3):362-367. | |
| Authors: | JIA Shu-juan ZENG Da-xing WU Xiao-li TU Guo-quan |
| Institution: | JIA Shu-juan1,2,ZENG Da-xing2,WU Xiao-li1,TU Guo-quan1(1.College of Bioscience and Bioengineering,Jiangxi Agricultural University,Nanchang 330045,2.School of Applied Chemistry and Biological Technology,Shenzhen Polytechnic,Shenzhen 518055,China) |
| Abstract: | Bacillus subtilis C-D6 and its culture filtrate showed obvious antifungal activity against Magnaporthe oryzae in dual plate.An antifungal protein(AFP),inhibitory to the formation of appressorium of M.oryzae,was isolated from C-D6 culture filtrate by using ammonium sulfate precipitation at 20% saturation and Sephadex G-75 column chromatography.SDS-PAGE analysis demonstrated that the protein was a monomer with molecular weight of 32 kD.Light microscopic observation showed that spore of M.oryzae treated with A... |
| Keywords: | Bacillus subtilis antifungal protein Magnaporthe oryzae appressorium |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |