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| 斜纹夜蛾抗菌物质的诱导及其理化特性 | |
| 引用本文： | 刘建涛,苏志坚,王方海,李广宏,宋少云,庞义.斜纹夜蛾抗菌物质的诱导及其理化特性[J].中国生物防治,2006,22(3):211-215. |
| 作者姓名： | 刘建涛 苏志坚 王方海 李广宏 宋少云 庞义 |
| 作者单位： | 1. 江西科技师范学院 2. 暨南大学药学院,广州,510632;暨南大学药学院,广州,510632 3. 暨南大学药学院,广州,510632 |
| 基金项目： | 国家自然科学基金;广东省科技攻关计划;广东省肇庆市科技计划 |
| 摘 要： | 用大肠杆菌K12D31免疫斜纹夜蛾六龄幼虫，12h后收集血淋巴并通过高温处理制成粗提液。SDS-PAGE电泳结果表明：诱导后粗提液含有3条分别为43．5、15．0、4．0kD的特异性条带。诱导产生的抗菌物质在酸性、高温和常温等条件下保持稳定。抗菌谱测定结果表明，诱导产生的抗菌物质对猪霍乱沙门氏菌、枯草芽孢杆菌、铜绿假单胞菌等革兰氏阴性和阳性菌有抑制作用，而且对白色念珠球菌、酿酒酵母也有抑制作用。 |
| 关 键 词： | 斜纹夜蛾 抗菌物质 抗菌谱 诱导 |
| 文章编号： | 1005-9261(2006)03-0211-05 |
| 收稿时间： | 2005-06-07 |
| 修稿时间： | 2005年6月7日 |
Induction and Characterization of Antibacterial Substance of Spodoptera litura |
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| LIU Jian-tao,SU Zhi-jian,WANG Fang-hai,LI Guang-hong,SONG Shao-yun,PANG Yi.Induction and Characterization of Antibacterial Substance of Spodoptera litura[J].Chinese Journal of Biological Control,2006,22(3):211-215. | |
| Authors: | LIU Jian-tao SU Zhi-jian WANG Fang-hai LI Guang-hong SONG Shao-yun PANG Yi |
| Institution: | Institute of Entomology, State Key Lab for Biocontrol, Zhongshan University, Guangzhou 510275, China |
| Abstract: | By injection of Escherichia coli K12D31 into 6th instar larvae of Spodoptera litura, antibacterial substances were induced in haemolymph of the larvae after 12 h. SDS-PAGE analysis indicated that there were three bands with molecular weight 43.5, 15.0 and 4.0 kD, respectively, in the antibacterial crude extract. Characterization of the antibacterial substances showed that the induced antibacterial substances kept high stability under acid condition, a short- time exposure to heating and room temperature. The antibacterial substances were also inhibitive to Candida albicans and Saceharomyces cerevisiae, besides Salmonella choleraesuis subsp, choleraesuis, Bacillus subtilis and Pseudomonas aeruginosa, and other bacteria. |
| Keywords: | Spodoptera litura antibacterial substances antibacterial spectrum induction |
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