| 内生细菌B_(47)菌株的鉴定及其对番茄青枯病的防效测定 |
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| 引用本文： | 黎起秦,叶云峰,蒙显英,彭好文,林纬,罗宽.内生细菌B_(47)菌株的鉴定及其对番茄青枯病的防效测定[J].中国生物防治,2005(3). |
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| 作者姓名： | 黎起秦 叶云峰 蒙显英 彭好文 林纬 罗宽 |
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| 作者单位： | 湖南农业大学，广西大学农学院，广西大学农学院，广西大学农学院，广西大学农学院，湖南农业大学 长沙410128，广西大学农学院，南宁530005，南宁530005，南宁530005，南宁530005，南宁530005，长沙410128 |
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| 基金项目： | 广西区自然科学基金资助项目(桂科基0009018) |
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| 摘 要： | 通过形态特征、生理生化特征和16SrDNA序列分析,对分离于番茄茎部能较强抑制番茄青枯病菌生长的内生细菌B47菌株进行了鉴定。结果表明,该菌为枯草芽孢杆菌,其最适生长pH5~6,最适生长温度为35℃。室内防治试验结果表明,用淋根法先接种B47菌后接种病原菌和用注射法先接种B47菌后接种病原菌的处理可取得81.25%和92.10%的防效,而用淋根法、注射法同时接种B47菌与病原菌的处理防效较低。
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| 关 键 词： | 内生细菌 鉴定 枯草芽孢杆菌 番茄青枯病 防治效果 |
Identification of Endophytic Bacterium Strain B_(47) and Its Control Effects on Tomato Bacterial Wilt |
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| LI Qi-qin,YE Yun-feng,MENG Xian-ying,PENG Hao-wen,LIN Wei,LUO Kuan.Identification of Endophytic Bacterium Strain B_(47) and Its Control Effects on Tomato Bacterial Wilt[J].Chinese Journal of Biological Control,2005(3). |
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| Authors: | LI Qi-qin YE Yun-feng MENG Xian-ying PENG Hao-wen LIN Wei LUO Kuan |
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| Abstract: | Tomato bacterial wilt is an important plant soilborne disease. The endophytic bacterium strain B_(47) isolated from stem of tomato could strongly inhibit the growth of .Ralstonia solanacearum.. This strain was identified as Bacillus subtilis according to its morphological, physiological and biochemical characteristics and 16S rDNA sequence analysis. The optimum pH and temperature for the growth of the strain were 5-6 and 35 respectively. The results of control tests in greenhouse showed that 81.25% and 92.10% of control effects on tomato bacterial wilt could be obtained when strain B_(47) was inoculated to tomato in advance of Ralstonia solanacearum. If strain B_(47) and Ralstonia solanacearum were inoculated to tomato simultaneously, the strain B_(47) could not effectively control the disease. |
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| Keywords: | endophytic bacterium identification Bacillus subtilis tomato bacterial wilt control effects |
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