格氏线虫表皮蛋白和分泌蛋白抑制昆虫免疫活性的比较

格氏线虫表皮蛋白和分泌蛋白都具有抑制昆虫免疫反应的能力,能够帮助侵染期线虫侵染寄主,但两者所含蛋白组分并不相同,从而导致蛋白活性也有所差异。本研究采用乙醇萃取和昆虫匀浆诱导,分别从侵染期的格氏线虫体表及分泌物中得到了表皮蛋白和分泌蛋白,并比较了这两者之间的异同。结果表明,无论是在昆虫体内还是体外,侵染期线虫表皮蛋白和分泌蛋白都具有抑制昆虫免疫反应的生物活性,并且分泌蛋白的活性更强。本研究为进一步研究线虫侵染与昆虫免疫间的相互关系和线虫抑制昆虫免疫反应的作用机理提供了科学佐证。

Comparison of the Immunosuppressive Activity between Cuticle Coat Proteins and Secrete Proteins from Steinernema glaseri

Both surface coat proteins （SCPs） and secrete proteins （SPs） from Steinernema glaseri infective juveniles are able to suppress insect immune activities and were proved to play important roles in promoting infection of entomopathogenic nematodes. However, they are different in activities because of their different constituents. In this study, the SCPs were extracted with ethanol and the SPs were induced by Galleria melonella homogenates, then the similarity and difference between them were compared. Result indicated that no matter in vivo or in vitro, both kinds of proteins have the ability to suppress insect immunity, but the activity of SPs is more powerful than that of SCPs. Our studies provide scientific evidence for further study of the relationship between entomopathogenic nematode and insect, and the immunosuppressive mechanism of Steinernema glaseri.