| 枯草芽孢杆菌Tpb55抗烟草普通花叶病毒活性研究 |
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| 引用本文： | 张成省,孔凡玉,刘朝科,王凤龙,张林,钱玉梅.枯草芽孢杆菌Tpb55抗烟草普通花叶病毒活性研究[J].中国生物防治,2008(Z1). |
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| 作者姓名： | 张成省 孔凡玉 刘朝科 王凤龙 张林 钱玉梅 |
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| 作者单位： | 中国农业科学院烟草所;山东农业大学;曲靖市烟草公司; |
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| 基金项目： | 中国农科院院长基金(2007); 国家烟草专卖局项目 |
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| 摘 要： | 利用三生-NN烟测定Tpb55菌悬液对烟草普通花叶病毒(TMV)钝化、预防和治疗作用结果表明,Tpb55菌悬液对TMV具有较好的钝化、预防和治疗效果,枯斑抑制率分别为96.15%、79.72%和65.71%。为进一步揭示Tpb55抗毒机理,选用易感TMV烟草品种K326为实验材料,研究以Tpb55对烟草花叶病毒(TMV)进行预防和治疗处理,测定烟草中与抗病毒性相关的部分生理生化指标的变化。结果表明,先用Tpb55菌液再接种毒液处理的烟株,对TMV的抑制作用优于先接种毒液再接种菌悬液处理,说明Tpb55对TMV的预防具有积极效果;不论在烟株接种毒液前或后,用Tpb55菌液处理烟株的叶片与只接种毒液的相比,叶片内过氧化物酶(POD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)活性均显著提高,以接毒前Tpb55菌液处理的烟株活性水平最高。从本试验结果来看,Tpb55具较强的抗TMV活性,可通过直接接触钝化作用及诱导烟草抗病性提高来抑制TMV侵染。
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| 关 键 词： | 烟草花叶病毒 枯草芽孢杆菌 生物防治 抗性诱导 |
Anti-TMV Activity of Bacillus subtilis Strain Tpb55 |
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| ZHANG Cheng-sheng,KONG Fan-yu,LIU Chao-ke,WANG Feng-long,ZHANG Lin,QIAN Yu-mei.Anti-TMV Activity of Bacillus subtilis Strain Tpb55[J].Chinese Journal of Biological Control,2008(Z1). |
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| Authors: | ZHANG Cheng-sheng KONG Fan-yu LIU Chao-ke WANG Feng-long ZHANG Lin QIAN Yu-mei |
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| Institution: | ZHANG Cheng-sheng~1,KONG Fan-yu~1,LIU Chao-ke~2,WANG Feng-long~1,ZHANG Lin~3,QIAN Yu-mei~1 (1.Tobacco Research Institute of CAAS,Qingdao 266101,2.Sh,ong Agricultural University,Taian 271018,China) |
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| Abstract: | Nicotiana tabacum var.Samsum NN was used to determine inactivation,protective,and curative efficacies of Tpb55 against tobacco mosaic virus(TMV).Tpb55 provided good inactivation,protective, and curative activity,with inhibition rates of 96.15%,79.72%and 65.71%respectively.In order to reveal its resistant mechanism of tobacco to TMV,some physiological and biochemical indexes in some physiological and biochemical indexes in tobacco were studied with Tpb55 protection and therapy treatment with the susceptible ... |
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| Keywords: | tobacco mosaic virus Bacillus subtilis biological control induced resistance |
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